Based on the Landsat 5 TM/Landsat 8 OLI images of Guilin City in 2002, 2012, and 2017, the objectoriented land use classification method and mathematical models (such as transfer matrix and dynamic degree), as well as the Chinese ecosystem service unit area value equivalent factor table, were used to quantifiably analyze the land use status and changes in the value of ecosystem services in Guilin from 2002 to 2017. The results showed that forest land was the main matrix type in the study area. During the study period, forest land and unused land were the types of land that had increased and decreased the most, respectively, while the reduced unused land was mainly transferred to forest land and cultivated land. The changes in unused land and construction land were particularly intense, and the land use changes of Guilin as a whole continued to increase during the study period. The value of ecosystem services in Guilin continued to rise, and the value of ecosystem services on forest land accounts for more than 85% of the entire ecosystem. The value of services such as soil formation and protection, water conservation, and biodiversity conservation were higher within the value of each individual service. In contrast, services such as food production had the lowest value, indicating that the service function of Guilin’s ecosystem was far greater than the productive function.