Due to their special qualities, nanoparticles are quite interesting. Various techniques have been developed for their synthesis, which frequently calls for high pressure, temperature, and solvents. Therefore, green synthesis may be the ideal choice as an affordable and environmentally responsible method for creating nanoparticles. A common strategy in material synthesis for environmental friendliness is the green production of nanoparticles employing various plant components and their extracts. Numerous metal nanoparticles with putative bioactivities have been produced using plant extracts. This study describes the synthesis of silver nanoparticles (AgNPs) using a combined extract of Onion and Garlic peel, as well as an evaluation of their cytotoxicity activities. AgNPs morphology and crystalline phase were characterized using UV spectroscopy, Fourier transform infrared spectroscopy, X-ray diffraction, and Scanning electron transfer microscopy analysis. The generated AgNPs were almost quasi-spherical in shape, well distributed and scattered in nature, and had an average size diameter of 10.5 nm, according to characterisation study. The AgNPs displayed unique cytotoxic properties. The outcomes demonstrated the potential of combined extract of Onion and Garlic peel extract as a biomaterial for the synthesis of AgNPs.