Culture-Dependent Analysis of 16S rRNA Sequences Associated with the Rhizosphere of Lemna minor and Assessment of Bacterial Phenol-Resistance: Plant/Bacteria System for Potential Bioremediation – Part II
Olga Radulovic 1  
Marija Petrić 2  
Martin Raspor 2  
Olja Stanojević 3  
Tamara Janakiev 3  
Vojin Tadić 4  
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Institute for Chemistry, Technology and Metallurgy, University of Belgrade, Njegoševa 12, Belgrade
Institute for Biological Research “Siniša Stanković”, University of Belgrade, Despota Stefana 142 Street, Belgrade, Serbia
Faculty of Biology, University of Belgrade, Studentski Trg 16, Belgrade, Serbia
Mining and Metallurgy Institute, Zeleni Bulevar 35, 19219 Bor, Serbia
Olga Radulovic   

Institute for Chemistry and Metallurgy, University of Belgrade, Njegoševa 12, Belgrade, Njegoseva 12, 11000 Belgrade, Serbia
Online publish date: 2018-10-08
Publish date: 2018-12-20
Submission date: 2017-11-27
Final revision date: 2017-12-26
Acceptance date: 2018-01-02
Pol. J. Environ. Stud. 2019;28(2):811–822
In this work, we demonstrate that the rhizosphere of common duckweed (Lemna minor) is inhabited with various phenol-resistant bacterial strains. Based on 16S rRNA sequencing, we have identified 60 rhizosphere-associated bacterial isolates belonging to 10 different bacterial genera (Pseudomonas, Hafnia, Serratia, Enterobacter, Micrococcus, Stenotrophomonas, Xanthomonas, Bacillus, Staphylococcus and Klebsiella). All isolates have been tested for phenol resistance and ability to utilize phenol as the sole carbon source. 70% of all isolates survived high doses of phenol (≥200 mg/L) and at least 27% can be potentially acclimatized by gradual increase of phenol concentration. Finally, based on high phenol resistance, ability to utilize phenol as the sole carbon source and documented low pathogenicity, we propose 5 strains as potentially excellent candidates for bioremediation. These 5 strains taxonomically correspond to Klebsiella sp., Serratia sp., and Hafnia sp., respectively. To the best of our knowledge, this is the first attempt to assess decontamination capacity of Serratia nematodiphila and Hafnia sp. in the context of bioremediation of phenol-contaminated aqueous media. Although additional analyses are needed, interaction between the common duckweed and the selected bacterial strains may be utilized in future bioremediation strategies.