Identification and Nitrogen Removal
Characteristics of a Denitrifying Strain

3/2013 vol. 22

ORIGINAL RESEARCH

Identification and Nitrogen Removal Characteristics of a Denitrifying Strain

Yan Ji^1,2, Haiyan Pei^1,2, Wenrong Hu^1,2

¹School of Environmental Science and Engineering, Shandong University,
²Shandong Provincial Engineering Center on Environmental Science and Technology,
Jinan 250061, Shandong Province, China

Pol. J. Environ. Stud. 2013;22(3):699-706

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KEYWORDS

denitrifying bacteria

ABSTRACT

The nitrogen (N) removal characteristics of denitrifying bacterium LZ-14 were investigated in this study. Extensive carbon sources can be used for LZ-14, with acetate as the most effective one. The strain LZ-14 grew fast at a range of 20ºC to 35ºC, and exhibited high N-removal efficiency. Nitrogen removal mainly was performed in the growth phase of 12-36 hr with an amount of nitrite accumulation that was reduced completely in the following 24 hr. The strain LZ-14 was identified as Pseudomonas by PCR amplification and homology analysis of 16S rDNA sequence. Strain LZ-14 was inoculated in simulative constructed wetlands (SCWs) to enhance pollutant removal in pilot-scale, which showed a significant effect on COD_Cr and total nitrogen (TN) removal.

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