Impact of Salinity Stress on Proline Reaction, Peroxide Activity, and Antioxidant Enzymes in Maize (Zea mays L.)
Davar Molazem, Ali Bashirzadeh
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Department of Agriculture Astara Branch, Islamic Azad University, Astara, Iran
Submission date: 2014-06-29
Final revision date: 2014-08-27
Acceptance date: 2014-09-03
Publication date: 2015-04-02
Pol. J. Environ. Stud. 2015;24(2):597–603
Salinity is one of the environmental limiting factors in agricultural production. For the aim of investigating the effects of salt stress on some physiological traits in eight varieties, including K3615/1, S.C704, B73, S.C302, Waxy, K3545/6, K3653/2, and Zaqatala in three salinity levels, Zero (control), 50, and 100 mM NaCl in three replicates for the factorial experiment was carried out in randomized complete block design. Treatments were planted in pots. During the experiment superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), chlorophyll a, chlorophyll b, LRWC, and proline were measured. Increasing salinity to 50 mM and 100 mM increased SOD in most varieties. Maximum SOD in 50 mM was seen in K3545/6 with 20.62 unit/min g fw, that with SC302 and B73 were no significant differences, but with other varieties had significant differences. In concentrations of 100 mM, there was no significant difference between varieties in ascorbate peroxidase (APX) enzyme. In these conditions maximum and minimum amount of (APX) was seen in Sc704 with 8.533 unit/min g fw and K3615.1 with 3.898 unit/min g fw, respectively. In concentrations of 100 mM, there was a significant difference between varieties in (CAT) enzyme. In these conditions maximum and minimum amounts of (CAT) were seen in Sc302 and Sc704, respectively. Between catalase with SOD (0.234) and proline (0.284) we found positive and significant correlation at the 5% level. Significant positive correlation between proline with ascorbate peroxidase, catalase, and superoxide dismutase were observed.