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ORIGINAL RESEARCH
Isolation and Bioinformatics Analysis
of SK2-type of Dehydrin1 (DHN1) Gene
from Egyptian Sorghum for Enhancing Salinity
Stress Tolerance in Transgenic Rice
1
Department of Biology, College of Science, Taif University, Taif, Saudi Arabia
2
Department of Plant Gene transfer, Agricultural Genetic Engineering Research Institute (AGERI),
Agricultural Research Center (ARC), 12619Giza Egypt
3
Department of Plant Molecular Biology, Agricultural Genetic Engineering Research Institute (AGERI),
Agricultural Research Center (ARC),12619 Giza Egypt
4
Department of Microbial Molecular Biology, Agricultural Genetic Engineering Research Institute (AGERI),
Agricultural Research Center (ARC), Giza Egypt
5
EPCRS Excellence Center, Plant Pathology and Biotechnology Laboratory, Agricultural Botany Department,
Faculty of Agriculture, Kafrelsheikh University, 33516, Egypt
Submission date: 2024-05-04
Final revision date: 2024-06-14
Acceptance date: 2024-07-19
Online publication date: 2024-11-13
Publication date: 2025-07-22
Corresponding author
Mahmoud A. Basry
Department of Plant Molecular Biology, Agricultural Genetic Engineering Research Institute (AGERI), Agricultural Research Center (ARC),12619 Giza Egypt
Department of Plant Molecular Biology, Agricultural Genetic Engineering Research Institute (AGERI), Agricultural Research Center (ARC),12619 Giza Egypt
Pol. J. Environ. Stud. 2025;34(5):5515-5525
KEYWORDS
TOPICS
ABSTRACT
To address the impact of salt stress on rice development and productivity, the Sorghum bicolor DHN1
(SbDHN1) gene was isolated and cloned into the pCambia1390 plant expression vector. Subsequently,
two rice cultivars, Giza 177 and Giza 178, were transformed with the SbDHN1 gene for improved salt
tolerance using biolistic transformation. The polymerase chain reaction (PCR) and reverse transcription
polymerase chain reaction (RT-PCR) techniques confirmed the successful incorporation of the SbDHN1
gene into the rice genome. PCR analysis revealed transformation rates of 13% and 14% for Giza 177
and Giza 178, respectively. Both semi-qRT-PCR and salt evaluation further confirmed the expression
of the SbDHN1 gene. The SbDHN1 sequence comparison with the published sequences in the NCBI
database revealed that the SbDHN1 gene is unique since it showed in amino acid sequences homology
ranging from 99.34-61.02% with the corresponding sequences in the database. In addition, the tolerance
of the transgenic rice to salinity indicates the protective effect of the SbDHN1 via a common cellular
pathway activated by salinity and enables it to grow better in marginal and newly reclaimed areas of Egypt. Nevertheless, the outcomes of this research pave the way for further improvement of various
rice genotypes and other cereal crops to meet the global demand for food and feed in Egypt.
CONFLICT OF INTEREST
The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
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