Methane-Oxidizing Microorganism Properties in Landfills
Juan Mei 1, 2, 3  
,   Yining Wu 1  
,   Feiyue Qian 1, 2  
,   Chongjun Chen 1, 2  
,   Yaoliang Shen 1, 2,   Youcai Zhao 4
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College of Environmental Science and Engineering, Suzhou University of Science and Technology, Suzhou, P.R. China
National and Local Joint Engineering Laboratory of Municipal Sewage Resource Utilization Technology, Suzhou, P.R. China
Jiangsu Collaborative Innovation Center of Technology and Material of Water Treatment, Suzhou, P.R. China
State Key Laboratory of Pollution Control and Resources Reuse, Tongji University, Shanghai, P.R. China
Juan Mei   

Suzhou University of Science and Technology, China, 1 Kerui Road, New District, Suzhou, China, 215009 Suzhou, China
Submission date: 2018-05-29
Final revision date: 2018-09-11
Acceptance date: 2018-09-23
Online publication date: 2019-05-28
Publication date: 2019-07-08
Pol. J. Environ. Stud. 2019;28(5):3809–3818
Bio-oxidation of methane in a landfill environment is important for mitigating global methane emissions into the atmosphere. In the present study, the characteristics of methane bio-oxidation and methane-oxidizing microorganisms were studied by enrichment cultivation using fresh and aged leachate (collected from Qizishan Landfill, Suzhou, China). Both aerobic and anaerobic methane oxidation were detected, methane oxidation capacities of the culture liquid were 5.24–7.26 μmol/mL/d under aerobic conditions and 4.41-3.70 μmol/mL/d under anaerobic conditions. The stoichiometry of anaerobic oxidation of methane (AOM) showed the complexity of AOM mechanisms in the leachate culture, with the types of sulfate-dependent, denitrification-dependent and iron-dependent AOM. The 16S rRNA gene sequence analysis and SEM analysis results showed that the genus Methylocystis was the dominant bacteria in aerobic cultures (relative abundance 35.96–78.37%). Genus Moheibacter (41.38%) and Cupriavidus (43.08%) were the most dominant taxa in anaerobic cultures, with aerobic methanotrophs Methylocaldum and Methylocystis in low abundance, and no anaerobic methane-oxidizing archaea (ANME) was found. Further research is needed to confirm whether aerobic methanotrophs can oxidize methane under anaerobic conditions.