ORIGINAL RESEARCH
Phthalic Acid Esters Increased Activation of Urease by Altering the Molecular Conformation
Chen Wenjing 1, 2, 3,   Hu Yunlong 1, 2,   Wang Zhigang 1, 2, 3  
,   Xu Weihui 1, 2
 
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1
Heilongjiang Provincial Technology Innovation Center of Agromicrobial Preparation Industrialization, Qiqihar, 161006, China
2
College of Life Sciences, Agriculture and Forestry, Qiqihar University, Heilongjiang Province, Qiqihar 161006, China
3
Center for Ecological Research, Northeast Forestry University, Heilongjiang Province, Harbin 150040, China
CORRESPONDING AUTHOR
Wang Zhigang   

Qiqihar University, China
Submission date: 2021-01-31
Final revision date: 2021-04-10
Acceptance date: 2021-04-15
Online publication date: 2021-09-10
 
 
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ABSTRACT
Phthalic acid esters (PAEs), including dimethyl phthalate (DMP) and dibutyl phthalate (DBP), were widely known by their potential biological toxicity. In this work, the interaction between PAEs and urease was studied by the resonance light scattering spectrum (RLS), average particle size, molecular docking, molecular dynamics simulation, enzyme activity assay and reaction kinetic experiment. Both DMP and DBP could form stable complexes with urease, and enhance the rigidity and stability of urease. The RLS and average particle size of urease were decreased by DMP and DBP. Moreover, the activation mechanism was non-competitive activation mechanism because Km of urease kept the same and Vmax were increased. It could be concluded that PAEs altered the molecular conformation of urease and increased the urease activity by the non-competitive activation mechanism.
eISSN:2083-5906
ISSN:1230-1485