Cr(III) removal ability of indigenous soil fungus of Pakistan (i.e. Rhizopus arrhizus Fisher) was checked through batch trails. Experiments were performed by taking 0.1 g of powdered fungal biomass in 100 mL of metal solution kept at 150 rpm for 3 hours. Results of FTIR spectroscopy revealed that amine (-NH₂) and hydroxyl (-OH) groups participated in Cr(III) binding with the fungal biomass. There was an inversely proportional relationship between adsorption efficiency and capacity on increasing initial metal concentration in the range of 20-100 mg/L. The adsorption capacity of fungal biomass was 43.47 mg/g as determined by Langmuir isotherm. High correlation coefficient (R²: 0.99) acquired through Langumir and Frendulich models adequately described the adsorption mechanism on fungal biomass. pH optimization trials in the range of 2-10 exhibited significantly greater adsorption efficiency of 75% at pH 4, while metal removal rate declined with increasing pH. Adsorption/adsorption trials with four acids indicted that maximum desorption of Cr(III) was found with HCl, followed by HNO₃, CH₃COOH, and H₂SO₄. Adsorption-based trials summarized that R. arrhizus is a potential and inexpensive biomaterial with viable application in the removal of Cr(III) from the aqueous solution.