In this study, a new soil-based microbial fuel cell was developed that can be used for biological remediation in areas with soil pollution. Electricity generation was studied with the developed soilbased microbial fuel cell. Voltage values were measured depending on time and the effects of sodium acetate at different concentrations (20, 40, 60, 80, 100 mM) were investigated. Our results showed that up to 396 mV electricity generation is possible with the new soil-based microbial fuel cell. The voltage values gradually increased with increasing sodium acetate concentration. The half-saturation constant was found to be 75.99 (R² = 0.97). In conclusion, the developed soil-based microbial fuel cell has shown that it has biosensor potential and can be used in the detection of various environmental pollutants.