Our work presents the results of the determination of bioavailable and total chromium (III) and chromium (VI) in the soil of an allotment garden situated in the center of Łódź. Chromium was determined by the spectrophotometric method in the form of a purple-red Cr (VI) complex with 1,5-diphenylcarbazide in the acid medium, following the establishment of the optimum amounts of the oxidant (ammonium peroxidisulphate (VI)) in the soil solutions. Moreover, the effects of iron and copper ions on the determination results were examined. For comparison, chromium was also determined by atomic absorption spectrometry.