This study focused on determining thallium content in tobacco samples collected from a commercial cigarette brand commonly available in Poland (manufactured by Philip Morris). The samples were analyzed with the use of differential pulse anodic stripping voltammetry (DPASV) coupled with a flow injection analysis (FIA) system. The obtained results confirmed that thallium was present in the studied tobacco samples (average content at 0.0089 ±0.0012μg/g). These findings suggest that cigarette smokers in Poland may be exposed to the toxic effects of thallium-based compounds present in tobacco.