The purpose of this research is to better understand the effect of different copper (Cu) levels of feed on the antioxidant capacity in the native sheep, solve the adverse effects of local seasonal Cu deficiency on sheep, and provide a reasonable reference for Cu supplementation. 10 healthy native sheep were selected as the control group (C group). 40 Cu-deficient native sheep were selected as tested group, and divided randomly into 4 groups (n = 10), supplied by CuSO4 (0, 20, 40, 60 mg/kg for group I, group II, group III and group IV, respectively). The results showed that the level of Cu, hemoglobin (Hb), packed-cell volume (PCV), albumin (ALB), the activities of ceruloplasmin (CP), Cu/Znsuperoxide dismutase (Cu/Zn-SOD) and catalase (CAT) in group I were significantly lower than those in C group, group II, III, IV (P<0.05), but malondialdehyde (MDA) concentration was higher. The activities of these antioxidant enzymes and MDA concentration had no significant difference between C group, group II, III, IV (P>0.05), but group III had the highest antioxidant enzymes activity and the lowest MDA concentration. There was no significant difference in manganese (Mn), zinc (Zn), iron (Fe), molybdenum (Mo), selenium (Se), red blood cell count (RBC), white blood cell count (WBC), blood urea nitrogen (BUN), alanine transaminase (ALT), alkaline phosphatase (ALP), aspartate aminotransferase (GOT), total cholesterol (TC), manganese-superoxide dismutase (Mn-SOD) between all groups (P>0.05). Conclusion: The function of antioxidant system of the native sheep in Weining County have seriously reduced. Added CuSO₄ to the diet increase the activity of antioxidant enzymes and improve the health parameters, but them did not increase linearly. Added 40 mg/kg CuSO₄, the activity of antioxidant enzymes was the highest and the content of MDA was the lowest. Therefore, it is recommended to add 40 mg/kg CuSO₄ to diet of the native sheep in Weining County.