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ORIGINAL RESEARCH
Influence of Different Habitats’ Seed and Their
Sizes on Germination, Seedling Growth
and Physiological Traits of a Medicinal
Plant Calotropis gigantea L.
1
Department of Crop Physiology and Ecology, Hajee Mohammad Danesh Science and Technology University,
Dinajpur-5200, Bangladesh
2
Department of Plant Resources, College of Industrial Sciences, Kongju National University,
Yesan 32439, Republic of Korea
3
Department of Agronomy, Hajee Mohammad Danesh Science and Technology University,
Dinajpur-5200, Bangladesh
These authors had equal contribution to this work
Submission date: 2024-09-11
Final revision date: 2024-12-08
Acceptance date: 2025-01-17
Online publication date: 2025-04-16
Publication date: 2026-04-21
Corresponding author
Md. Rabiul Islam
Department of Crop Physiology and Ecology, Hajee Mohammad Danesh Science and Technology University, Dinajpur-5200, Dinajpur, Bangladesh
Department of Crop Physiology and Ecology, Hajee Mohammad Danesh Science and Technology University, Dinajpur-5200, Dinajpur, Bangladesh
Pol. J. Environ. Stud. 2026;35(2):1657-1669
KEYWORDS
TOPICS
ABSTRACT
Habitat and seed size are the key factors that affect plant growth and development. The influence
of these factors on the life history traits of the wild medicinal Calotropis gigantea is scanty around
the globe. Hence, a laboratory and a pot experiment were conducted to investigate the impact of
various sources (habitats) of seeds and their sizes on germination, seedling growth, and physiological
traits of C. gigantea following a two-factor, completely randomized design with three repetitions.
Factor-A comprised five habitats’ seeds (T1-roadside, T2-railway line, T3-river bank, T4-waste dump,
and T5-grazing land), and factor-B consisted of three sizes (mass) of seeds (L-large, M-medium, and
S-small). In this study, we observed that the grazing land habitats’ seed (T5) performed better than other
sources of seeds for various studied traits. The performances of different sizes of seeds were categorized
as L > M > S. Overall, it was notable that the greater performances of different habitats’ seeds with
larger sizes were characterized as T5 × L > T1 × L > T2 × L > T3 × L > T4 × L for germination, seedling
growth, and physiological attributes. The present findings might have provided greater insights into the
growth, development, and productivity of C. gigantea useful to farmers, practitioners, and researchers.
CONFLICT OF INTEREST
The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
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