ORIGINAL RESEARCH
Synthesis of Silver Nanoparticle from Allium sativum as an Eco-Benign Agent for Biological Applications
 
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1
Department of Basic Sciences, University of Veterinary and Animal Sciences, Lahore (Jhang-Campus), Pakistan
 
2
Deputy Director, Livestock and Dairy Development Department, Punjab, Tehsil Shorkot, District Jhang, Pakistan
 
3
Department of Pathobiology, University of Veterinary and Animal Sciences, Lahore, (Jhang-Campus), Pakistan
 
4
Department of Biochemistry, University of Agriculture, Faisalabad, Pakistan
 
5
Department of Environmental Sciences, COMSATS University Islamabad, Vehari Campus, Punjab, Pakistan
 
6
Department of Biology, College of Sciences, Princess Nourah bint Abdulrahman University (PNU), Riyadh 11671, Saudi Arabia
 
7
Department of Chemistry, The University of Lahore, Lahore, Pakistan
 
 
Submission date: 2020-12-14
 
 
Final revision date: 2021-04-08
 
 
Acceptance date: 2021-04-13
 
 
Online publication date: 2021-12-22
 
 
Publication date: 2022-01-28
 
 
Corresponding author
Arif Nazir   

Department of Chemistry, The University of Lahore, Lahore, Lahore, Pakistan
 
 
Pol. J. Environ. Stud. 2022;31(1):533-538
 
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ABSTRACT
The study focuses on the fabrication of silver nanoparticles (Ag NPs) using aqueous extracts of Allium sativum as reducing agents. Silver ion becomes a good carrier of bioactive compounds of Allium sativum, so their biochemical potential such as total phenolic and flavonoids were evaluated. Results showed that as a concentration increases TPC and TFC were also increased. Similar to their secondary metabolite, scavenging potential of Ag NPs of Allium sativum was also monitored. Green synthesized Ag NPs exhibited significant antibacterial activity 24±2.6, 22±1.4, 19±2.7 and 20±3.17 against B. subtilis, S. aureus, E. coli and P. multocida bacterial strains respectively. Moreover, Ag NPs of garlic did not show any mutagenicity against mutant strain of S. typhimurium TA98 & TA100. Brine shrimp lethality assay (BSLA) showed their dose dependent effect. Finally, green synthesized Ag NPs of garlic presented anti-proliferative effect against HEPG2 cell line.
CONFLICT OF INTEREST
The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
 
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16.
Biosynthesis and characterization of silver nanoparticles using strawberry seed extract and evaluation of their antibacterial and antioxidant activities
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Journal of Saudi Chemical Society
 
eISSN:2083-5906
ISSN:1230-1485
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