In vitro Screening of Multifunctional Growth Stimulating Rhizobacteria Using Zea mays L.
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Department of Botany, University of the Punjab, Quaid-e-Azam Campus, Lahore 54590, Pakistan
Submission date: 2021-01-05
Final revision date: 2021-04-06
Acceptance date: 2021-04-27
Online publication date: 2021-10-19
Publication date: 2021-12-02
Corresponding author
Ambreen Ahmed   

Department of Botany, University of the Punjab, Department of Botany, 54590, Lahore, Pakistan
Pol. J. Environ. Stud. 2021;30(6):5559-5566
Present study highlights an organic scheme to achieve ameliorated growth of plants with enhanced nutritional status using plant growth promoting rhizobacteria (PGPR). Bacteria were in vitro screened for multifarious growth stimulatory traits including auxin production, ACC-deaminase activity, nitrogen fixation and ammonification, cyanide production and mineral solubilization. These traits are considered as the hallmarks for bacterial interaction with plants. Four efficient isolates Enterobacter sp. (A5C), Exiguobacterium sp. (A13G), Tr1 and Tr2 were selected on the basis of these traits and further applied on PGPR treated plant assay. Results revealed that all bacterial treatments significantly improved the growth parameters. Isolates Enterobacter sp. (A5C) and Tr1 tended to elevate chlorophyll ‘a’ upto 203.1 and 110.4% over control, chlorophyll ‘b’ was enhanced by 185.5 and 91.1% over control with application of Enterobacter sp. (A5C) and Exiguobacterium sp. (A13G) while isolates Tr2 and Tr1 tremendously increased carotenoid content upto 307.3 and 235.3% respectively, over control. Soluble sugar and free aminoacid content was increased up to 66 and 75.6% respectively, when inoculation was done with isolate Tr2. Similarly, isolate Tr1 caused 35.1, 385.4 and 109.4% increase in protein, phenolic and total alkaloid content respectively, when compared with control. Concerning these significant enhancements in physio-chemical traits of Zea mays plants, PGPR can be used as bioinoculants in agricultural systems for organic treatment to get maximum plant productivity in terms of their nutritional status and biochemistry which can help us to minimize input of synthetic chemicals for growth improvement thus saving the environment from increased soil and water pollution.
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