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ORIGINAL RESEARCH
Enhanced Plant Growth and Pathogen Inhibition
by Bacillus amyloliquefaciens Strain YB1701:
A Novel PGPR from Extreme Environments
1
College of Life Science, Shenyang Normal University, Shenyang 110034, China
Submission date: 2024-08-20
Final revision date: 2024-09-17
Acceptance date: 2024-10-13
Online publication date: 2025-01-29
Publication date: 2025-11-04
Pol. J. Environ. Stud. 2025;34(6):7909-7920
KEYWORDS
TOPICS
ABSTRACT
Bacteria that thrive in extreme environmental conditions possess unique abilities to promote plant
growth and enhance disease resistance. In this study, a plant growth-promoting rhizobacteria (PGPR)
was isolated and purified from the rhizosphere soil collected at the Red Beach of Panjin, designated as
strain YB1701. Strain YB1701 was identified as Bacillus amyloliquefaciens, a gram-negative Bacillus,
measuring between 1.6 μm to 3.1 μm in length and 0.9 μm to 1.1 μm in width. The optimal pH for strain
YB1701 growth was determined to be between 7.0 and 8.0, and the strain exhibited a strong ability to
degrade starch. The indole-3-acetic acid (IAA) content produced by the strain was 137.58 μg·mL-1, and
it showed 1-aminocyclopropane-1-carboxylate (ACC) deaminase activity of 3.03 μmol·mg-1·h-1. Strain
YB1701 inhibited 16 different plant pathogenic fungi by at least 50%, with more than 80% inhibition
observed against Exserohilum turcicum and Sclerotinia sclerotiorum. Additionally, treatment with
strain YB1701 significantly promoted the growth of rice seedlings, increasing shoot height, root length,
and shoot and root dry weight by 64.54%, 20.39%, and 71.94%, respectively. Root dry weight alone
increased by 2.36%. These findings suggest that B. amyloliquefaciens strain YB1701 has potential
applications in agriculture and other fields as a novel biocontrol agent and growth promoter. This strain
could enrich microbial species resources and provide a basis for the utilization of bacterial resources in
various applications.
CONFLICT OF INTEREST
The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
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