An Eco-Friendly Approach for the Extraction of Antioxidant Components from Artemisia Annua Leaves Using Response Surface Methodology
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Department of Chemistry, University of Sargodha, Sargodha 40100, Pakistan
Department of Chemistry, The University of Lahore, Lahore, Pakistan
Department of Chemistry, University of Education Lahore, Jauharabad Campus, Pakistan
Division of Science and Technology, Faculty of Science, University of Education, Lahore, Pakistan
Department of Chemistry, Government College University Lahore, Lahore, Pakistan
Umer Younas   

Department of Chemistry, The University of Lahore, Lahore, Lahore, Pakistan
Submission date: 2020-08-21
Final revision date: 2021-01-06
Acceptance date: 2021-01-25
Online publication date: 2021-08-30
Publication date: 2021-09-22
Pol. J. Environ. Stud. 2021;30(5):4827–4833
Drying methods, extraction techniques, solvents as well as extraction conditions affect the recovery of antioxidant components from botanical materials. In the current study, antioxidant compounds from leaf samples of A. annua were extracted using methanol as extraction solvent and orbital shaker assisted extraction technique and extraction technique was optimized using response surface methodology. Extraction conditions including rotation speed and extraction time were taken as independent variables and extraction was carried out for different time duration and at different rotation speed. A significant mutual effect of selected extraction conditions was recorded on the antioxidant potential of A. annua leaves and optimized conditions were determined employing response surface methodology. Statistical data revealed linear quadratic and interactive effect of extraction conditions on TPC, TFC and DDPH radical scavenging potential was significant because p-value is less than 0.05. For the same antioxidant components, measured and predicted values were found to be close in agreement, indicating that the tested model is fit for the recovery of antioxidant components from A. annua leaves. Optimization of extraction conditions using response surface methodology may be helpful in making the extraction technique cost-effective and environment friendly.