ORIGINAL RESEARCH
Chemical Composition and in vitro Evaluation
of Cytotoxicity, Antioxidant and Antimicrobial
Activities of Essential Oil Extracted from Myristica
Fragrans Houtt
| 1 | Department of Chemistry, Govt. Postgraduate College of Science, Faisalabad, Pakistan |
| 2 | Department of Allied Health Sciences, The University of Lahore, Gujrat Campus, Pakistan |
| 3 | Department of Chemistry, The University of Lahore, Lahore, Pakistan |
| 4 | Department of Chemistry, University of Sahiwal, Sahiwal, Pakistan |
| 5 | Institute of Microbiology, University of Agriculture, Faisalabad, Pakistan |
| 6 | Division of Science and Technology, Department of Zoology, University of Education Lahore,
Multan Campus, Pakistan |
| 7 | Department of Biochemistry/Centre for Advanced Studies in Agriculture and Food Security (USPCASAFS),
University of Agriculture, Faisalabad, Pakistan |
| 8 | Division of Science and Technology, Department of Chemistry, University of Education Lahore,
Multan Campus, Pakistan |
| 9 | Department of Biotechnology, Lahore College for Women University, Lahore, Pakistan |
CORRESPONDING AUTHOR
Submission date: 2020-02-07
Final revision date: 2020-06-23
Acceptance date: 2020-06-27
Online publication date: 2020-12-03
KEYWORDS
TOPICS
ABSTRACT
This paper focuses on the chemical composition, antioxidant, cytotoxicity (brine shrimp lethality
and hemolytic assay) and antimicrobial activities of essential oil of mace (Myristica fragrans) oil. We
have found 33 compounds as evidenced by GC-MS analysis. The most prominent of these compounds
are safrole, β-phellandrene, 3-p-menthene and L-terpinen-4-ol. Antioxidant activity of the mace
essential oil was examined using the free radical scavenging assay (21.95 μg/mL), β-carotene in linoleic
acid and percent inhibition in linoleic acid (67.9 %) system. Four pathogenic fungi, 4 gram positive and
2 gram negative bacteria were used for antimicrobial activity of oil. Aspergillus niger and Streptococcus
mutans proved to be most sensitive with MIC values i.e. 1.01 and 1.72 mg/mL, respectively. The mace essential oil exhibited toxicity with LC50 value 31.05 μg/mL cytotoxicity against nauplii of Artemia
salina and minimum hemolytic activity was recorded as 5.21% at 0.5 mg/mL concentration.
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