ORIGINAL RESEARCH
Distribution of Microbial-Selected Populations in Lake North Mamry by Fluorescent in situ Hybridization
A. Skowronska
Department of Environmental Microbiology, Faculty of Science and Fisheries, University of Warmia and Mazury, ul. Prawochenskiego 1,10-957 Olsztyn-Kortowo, Poland
Pol. J. Environ. Stud. 2007;16(1):123–128
KEYWORDS
rRNA-targeted oligonucleotide probesfluorescent in situ hybridizationbacterial community composition
ABSTRACT
Fluorescent in situ hybridization (FISH) with rRNA -targeted oligonucleotide probes has become one of the major techniques in environmental microbiology, allowing rapid and reliable definition of prokaryotes and quantification of population sizes. The aim was to demonstrate the applicability of the FISH method to study bacterioplankton composition in North Mamry Lake, and to follow the dynamics of two populations of common bacteria. We analyzed the phylogenetic composition of free-living bacterioplankton assemblage using oligonucleotide probes specific for Bacteria as well as for β-Proteobacteria and Cytophaga-Flavobacterium groups. Up to 53% of bacteria detected with DAPI could be detected via FISH by applying the universal bacterial probe for domain Bacteria (Eub338). Percentage of Cytophaga-Flavobacterium cluster did not exceed 20%. Members of the β–Proteobacteria appeared to be the most abundant group.
We process personal data collected when visiting the website. The function of obtaining information about users and their behavior is carried out by voluntarily entered information in forms and saving cookies in end devices. Data, including cookies, are used to provide services, improve the user experience and to analyze the traffic in accordance with the Privacy policy. Data are also collected and processed by Google Analytics tool (more).
You can change cookies settings in your browser. Restricted use of cookies in the browser configuration may affect some functionalities of the website.
You can change cookies settings in your browser. Restricted use of cookies in the browser configuration may affect some functionalities of the website.