ORIGINAL RESEARCH
Effects of EMFs on Some Biological Parameters
in Coffee Plants (Coffea arabica L.) Obtained
by in vitro Propagation
Elizabeth Isaac Alemán1, Andrew Mbogholi2, Yilan Fung Boix1,
Justo González-Olmedo2, Antonio Chalfun-Junior3
1Departament of Bioelectromagnetism, Nacional Centre of Applied Bioelectromagnetism,
Apartado 4078, CP 90400, Santiago de Cuba, Cuba
2Department of Tissue Culture, Centre of Bioplants,
Km 9. Carretera Morón, Ciego de Avila, Cuba
3Department of Biology, Laboratory of Plant Molecular Physiology, Federal University of Lavras.
Campus Universitário, Lavras, Minas Gerais, Brasil
Apartado 4078, CP 90400, Santiago de Cuba, Cuba
2Department of Tissue Culture, Centre of Bioplants,
Km 9. Carretera Morón, Ciego de Avila, Cuba
3Department of Biology, Laboratory of Plant Molecular Physiology, Federal University of Lavras.
Campus Universitário, Lavras, Minas Gerais, Brasil
Pol. J. Environ. Stud. 2014;23(1):95–101
KEYWORDS
ABSTRACT
In vitro coffee seedlings were exposed to an electromagnetic field (EMF) of 2 mT during 3 minutes in
establishment, multiplication, and acclimazation phases. Shoot and root lengths and leaf pair numbers of treated
groups increased when compared to control; in addition to SOD, CAT, and APX activities of in vitro-treated
groups showed a decrease in levels. Four months after the magnetic treatment was applied, the same parameters
were evaluated. Shoot lengths, root lengths, the pair of leaves numbers, and CAT activity increased in
treated plants. APX activity decreased in treatment seedlings, whereas SOD activity did not show a difference
between experimental groups.
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