ORIGINAL RESEARCH
Enhancing Profitability of Ethanol Fermentation
through Gamma Ray Mutagenesis
of Saccharomyces cerevisiae
| 1 | Jhang-Campus, University of Veterinary and Animal Sciences Lahore, Pakistan |
| 2 | Poultry Research Institute Rawalpindi, Pakistan |
| 3 | Institute of Pharmacy, Physiology and Pharmacology, University of Agriculture Faisalabad Pakistan |
| 4 | Livestock and Dairy Development, Punjab Pakistan |
| 5 | Department of Chemistry, Karakoram International University, Gilgit-Baltistan, Pakistan |
| 6 | Department of Chemistry, The University of Lahore, Lahore-Pakistan |
| 7 | Department of Bioinformatics and Biotechnology, Government College University Faisalabad, Pakistan |
CORRESPONDING AUTHOR
Submission date: 2017-04-24
Final revision date: 2017-10-17
Acceptance date: 2017-10-17
Online publication date: 2018-08-06
Publication date: 2018-11-20
Pol. J. Environ. Stud. 2019;28(1):35–41
KEYWORDS
TOPICS
ABSTRACT
Invertase has a vital role in ethanol fermentation, especially on sucrose-containing media. In this
study, catabolite-resistant mutants of Saccharomyces cerevisiae were isolated through gamma ray
mutagenesis with improved productivity of ethanol and invertases. Enzyme productivity of mutant
yeast was compared with that of the parent. Various fermentation parameters were optimized, including
substrate concentration, pH, and temperature on the production of invertase and ethanol. Applying
optimized conditions (pH 4.6, 37ºC) at lab scale, industrial scale experiments in 300 m3 fermenters were
performed. Mutants had completed fermentation with high efficiency in 28 hrs irrespective of parents that
took 36 hrs. The mutant obtained is of great commercial value as it has the potential to enhance the overall
profitability of the fermentation process through advancement in the quality of the final product.
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