ORIGINAL RESEARCH
Enhancing Profitability of Ethanol Fermentation through Gamma Ray Mutagenesis of Saccharomyces cerevisiae
 
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1
Jhang-Campus, University of Veterinary and Animal Sciences Lahore, Pakistan
2
Poultry Research Institute Rawalpindi, Pakistan
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Institute of Pharmacy, Physiology and Pharmacology, University of Agriculture Faisalabad Pakistan
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Livestock and Dairy Development, Punjab Pakistan
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Department of Chemistry, Karakoram International University, Gilgit-Baltistan, Pakistan
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Department of Chemistry, The University of Lahore, Lahore-Pakistan
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Department of Bioinformatics and Biotechnology, Government College University Faisalabad, Pakistan
Online publish date: 2018-08-06
Publish date: 2018-11-20
Submission date: 2017-04-24
Final revision date: 2017-10-17
Acceptance date: 2017-10-17
 
Pol. J. Environ. Stud. 2019;28(1):35–41
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ABSTRACT:
Invertase has a vital role in ethanol fermentation, especially on sucrose-containing media. In this study, catabolite-resistant mutants of Saccharomyces cerevisiae were isolated through gamma ray mutagenesis with improved productivity of ethanol and invertases. Enzyme productivity of mutant yeast was compared with that of the parent. Various fermentation parameters were optimized, including substrate concentration, pH, and temperature on the production of invertase and ethanol. Applying optimized conditions (pH 4.6, 37ºC) at lab scale, industrial scale experiments in 300 m3 fermenters were performed. Mutants had completed fermentation with high efficiency in 28 hrs irrespective of parents that took 36 hrs. The mutant obtained is of great commercial value as it has the potential to enhance the overall profitability of the fermentation process through advancement in the quality of the final product.
CORRESPONDING AUTHOR:
Arif Nazir   
University of Lahore, Lahore, 1-Km Raiwind Road, Lahore, 54000 Lahore, Pakistan
eISSN:2083-5906
ISSN:1230-1485