About the Journal
In Memory of Professor Jerzy Radecki
Editorial Office
Editorial Board
Scope
Impact Factor
Indexed in...
Journal Impact Factor contributing items
Contact
Subscription
Current issue
Online first
Notes to Authors
Frequently Asked Questions
Editorial policies
PJOES Publication Policy Overview
PJOES Peer Review Policy
PJOES Research Ethics and Malpractice Policy
PJOES Plagiarism Policy
PJOES Conflict of Interest Policy
PJOES Open Access Policy
PJOES Instructions for Reviewers
Generative AI and AI-Assisted Technologies Policy
Archive
ORIGINAL RESEARCH
Toxicological Assessment of Aluminum Sulfate
and Three Mitochondrial Respiratory
Chain Inhibitors (Trimetazidine, Prednisolone,
and Potassium cyanide) on Yeast
Saccharomyces cerevisiae
1
Laboratory of Cell Toxicology, Department of Biology, Faculty of Sciences, University of Annaba
2
Environmental Research Center, University of Annaba
3
Toxicology and ecosystems pathologies Laboratory, Larbi Tebessi university, Algeria
Submission date: 2024-04-19
Final revision date: 2024-05-25
Acceptance date: 2024-06-28
Online publication date: 2024-09-10
Publication date: 2025-06-06
Corresponding author
Pol. J. Environ. Stud. 2025;34(4):4733-4740
KEYWORDS
TOPICS
ABSTRACT
Aluminum (Al) is a widely found metal with no known biological or clinical benefits, and thus
causes considerable toxicological effects on biological organisms’ health, including yeast.
Mitochondria are the main cellular organelles involved in the production of oxygen and reactive oxygen
species (ROS) in the cell. Yeast is the most scientifically used micro-ecologic, and the most sensitive
microorganisms to various toxicants. As the toxicological effects of Al and mitochondrial respiratory
chain inhibitors on Saccharomyces cerevisiae have not been elucidated; the present study
was therefore, devoted to comparing the toxic effects of Al salts and three mitochondrial inhibitors,
namely trimetazidine, Prednisolone, and Potassium Cyanide on Saccharomyces cerevisiae. Cells were
exposed 2h increasing concentrations of Al (C1 = 0.017g/L, C2 = 0.034g/L, C3 = 0.342 g/L,
and C4 = 8,5 g/L), and one concentration of mitochondrial inhibitor C1 = 0.0032g/L Potassium Cyanide
(KCN), C1 = 0.013 g/L Trimetazidine (TMZ), and C1 = 0.018 g/L Prednisolone (PDN). Results showed
an inhibition of cell growth in Al2 (SO4)3 and the used mitochondrial inhibitors, in particular, Cyanide
and Prednisolone as evidenced by positive response percentages in exposed yeasts. Moreover, oxidative
stress induction in Aluminum sulfate treatment was revealed by stimulation of catalase (CAT)
and Glutathione S-transferase (GST) activity, along with increased levels of GSH and MDA compared
with control.
CONFLICT OF INTEREST
The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
REFERENCES (56)
1.
BRYLIŃSKI Ł., KOSTELECKA K., WOLIŃSKI F., DUDA P., GÓRA J., GRANAT M., FLIEGER J., TERESIŃSKI G., BUSZEWICZ G., SITARZ R., BAJ J. Aluminium in the Human Brain: Routes of Penetration, Toxicity, and Resulting Complications. International Journal of Molecular Science, 24, 7228, 2023. https://doi.org/10.3390/ijms24... PMid:37108392 PMCid:PMC10139039.
2.
BOROTOVA P., DROBNY M., NAVRATILOVA A., POZGAJOVA M. Cytotoxic effect of aluminium ions on unicellular eukaryotic organism. Acta fytotechn zootechn, 22 (4), 2019. https://doi.org/10.15414/afz.2....
3.
BATISSE E. L'aluminium, un produit dangereux pour la santé présent en thérapeutique et en cosmétique : mythe ou réalité?, Thèse de doctorat, Université de Loraine, France, 2014.
4.
MIRZA A., KING A., TROAKES C., EXLEY C. Aluminium in brain tissue in familial Alzheimer's disease. Journal of Trace Elements in Medicine and Biology, 40, 30, 2017. https://doi.org/10.1016/j.jtem... PMid:28159219.
5.
CAPRIELLO T., DI MEGLIO G., DE MAIO A., SCUDIERO R., BIANCHI A.R., TRIFUOGGI M., TOSCANESI M., GIARRA A., FERRANDINO I. Aluminium exposure leads to neurodegeneration and alters the expression of marker genes involved to parkinsonism in zebrafish brain. Chemosphere, 307 (1), 135752, 2022. https://doi.org/10.1016/j.chem... PMid:35863414.
6.
IGBOKWE I.O., IGWENAGU E., IGBOKWE N.A. Aluminium toxicosis: a review of toxic actions and effects. Interdisciplinary toxicology, 2, 45, 2019. https://doi.org/10.2478/intox-... PMid:32206026 PMCid:PMC7071840.
7.
RAHIMZADEH M.R., RAHIMZADEH M.R., KAZEMI S., AMIRI R.J., PIRZADEH M., MOGHADAMNIA A.A. Aluminum Poisoning with Emphasis on Its Mechanism and Treatment of Intoxication. Emergency medicine international, 2022, 1480553, 2022. https://doi.org/10.1155/2022/1... PMid:35070453 PMCid:PMC8767391.
8.
BENYETTOU B., KHAROUBI O., HALLAL N., BENYETTOU H.A., TAIR K., BELMOKHTAR M., AOUES A., OZASLAN M. Aluminium-Induced Behavioral Changes and Oxidative Stress in Developing Rat Brain and the Possible Ameliorating Role of Omega-6/Omega-3 Ratio. Journal of Biological Sciences, 17, 106, 2017. https://doi.org/10.3923/jbs.20....
9.
ABDALLA R.P., KIDA B.M.S., PINHEIRO J.P.S., OLIVEIRA L.F., MARTINEZ C.B.F., MOREIRA R.G. Exposure to aluminum, aluminum + manganese and acid pH triggers different antioxidant responses in gills and liver of Astyanax altiparanae (Teleostei: Characiformes: Characidae) males. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 215, 33, 2019. https://doi.org/10.1016/j.cbpc... PMid:30315923.
10.
CHEN R., ZHU Q., FANG Z., HUANG Z., SUN J., PENG M., SHI P. Aluminium induces oxidative damage in Saccharomyces cerevicia. Canadian Journal of Microbiology, 66 (12), 713, 2020. https://doi.org/10.1139/cjm-20... PMid:32730711.
11.
REDDAM A., MCLARNAN S., KUPSCO A. Environmental Chemical Exposures and Mitochondrial Dysfunction: a Review of Recent Literature. Environmental Epigenetics, 631, 2022. https://doi.org/10.1007/s40572... PMid:35902457 PMCid:PMC9729331.
12.
KARATHIA H., VILAPRINYO E., SORRIBAS A., ALVES R. Saccharomyces cerevisiae as a model organism: a comparative study. PLoS One, 6 (2), 16015, 2011. https://doi.org/10.1371/journa... PMid:21311596 PMCid:PMC3032731.
13.
MOHAMMADI S., SABERIDOKHT B., SUBRAMANIAM S., GRAMA A. Scope and limitations of yeast as a model organism for studying human tissue-specific pathways. BMC Systems Biology, 29 (9), 96, 2015. https://doi.org/10.1186/s12918... PMid:26714768 PMCid:PMC4696342.
14.
ALLAF M.M., TRICK C.G. Yeast Cell as a Bio-Model for Measuring the Toxicity of Fish-Killing Flagellates. Toxins (Basel), 13 (11), 821, 2015. https://doi.org/10.3390/toxins... PMid:34822605 PMCid:PMC8623749.
15.
SOUSA C.A., SOARES H.M.V.M., SOARES E.V. Metal(loid) oxide (Al2O3, Mn3O4, SiO2 and SnO2) nanoparticles cause cytotoxicity in yeast via intracellular generation of reactive oxygen species. Applied Microbiology and Biotechnology, 103, 6257, 2019. https://doi.org/10.1007/s00253... PMid:31152204.
16.
LASSERRE J.P., DAUTANT A., AIYAR R.S., KUCHARCZYK R., GLATIGNY A., TRIBOUILLARDTANVIER D., RYTKA J., BLONDEL M., SKOCZEN N., REYNIER P., PITAYU L., RÖTIG A., DELAHODDE A., STEINMETZ L.M., DUJARDIN G., PROCACCIO V., DI RAGO J.P. Yeast as a system for modeling mitochondrial disease mechanisms and discovering therapies. Disease models & mechanisms, 8 (6), 509, 2015. https://doi.org/10.1242/dmm.02... PMid:26035862 PMCid:PMC4457039.
17.
IGHODARO O.M., IGHOdARO O.M., AKINLOYE O.A. First defence antioxidants-superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX): Their fundamental role in the entire antioxidant defence grid. Alexandria Journal of Medicine, 54, 287, 2018. https://doi.org/10.1016/j.ajme....
18.
YU Y., DI TRAPANI G., TONISSEN K.F. Thioredoxin and Glutathione Systems. Ed: Chakraborti S., Ray B.K., Roychoudhury S. Handbook of Oxidative Stress in Cancer: Mechanistic Aspects. Springer, 2407, 2022. https://doi.org/10.1007/978-98....
19.
GUNDERSON M.P., NGUYEN B.T., CERVANTES REYES J.C., HOLDEN L.L., FRENCH J.M.T., SMITH B.D., LINEBERGER C. Response of phase I and II detoxification enzymes, glutathione, metallothionein and acetylcholine esterase to mercury and dimethoate in signal crayfish (Pacifastacus leniusculus). Chemosphere, 208, 749, 2018. https://doi.org/10.1016/j.chem... PMid:29902759 PMCid:PMC6074053.
20.
HUANG Y., CHEN H., LIU Q., HU J., HU D., HUANG Z., XU Z., WAN R. Obesity difference on association blood malondialdehyde level and diastolic hypertension in the elderly population: a cross-sectional analysis. European Journal of Medical Research, 28 (44), 2023. https://doi.org/10.1186/s40001... PMid:36694211 PMCid:PMC9872357.
22.
CHERAIT A. Toxicity evaluation at the cellular and subcellular level of a compound from the dihydropyridine family on an experimental stress bioindicator model. Doctoral thesis, University of Annaba, Algeria, 2015.
23.
KHEBBEB M. Evaluation of the toxicity induced by ZnO nanoparticles on two unicellular biological models Saccharomyces cereviciae and Paramecium sp. Doctoral thesis, University of Annaba, Algeria, 2016.
24.
PROKIĆ M., BORKOVIĆ-MITIĆ S., KRIZMANIĆ I., GAVRIĆ J., DESPOTOVIĆ S., GAVRILOVIĆ B., RADOVANOVIĆ T., PAVLOVIĆ S., SAIČIĆ Z. Comparative study of oxidative stress parameters and acetylcholinesterase activity in the liver of Pelophylax esculentus complex frogs. Saudi Journal of Biological Sciences, (1), 51, 2017. https://doi.org/10.1016/j.sjbs... PMid:28053571 PMCid:PMC5198921.
25.
SIMARANI K., YUSOFF H.A.C., ALIAS Z. Purification of Glutathione Transferases (GSTs) from Identified Rhizospheric Bacteria. Sains Malaysiana, 45 (7), 1057, 2016.
26.
DAHDOUH F., BENDJEFFAL H., NOUACER Z., MOUMENE W., ZEMINOUR M.E.H., NAOUS M., DJEBAR H. Selenium Nanoparticles Attenuate Gentamycin-Induced Nephrotoxicity and Hematotoxicity in Female Swiss Albino Mice. BioNanoScience, 9, 356, 2019. https://doi.org/10.1007/s12668....
27.
GARCIA D., LIMA D., DA SILVA D.G.H., DE ALMEIDA E.A. Decreased malondialdehyde levels in fish (Astyanax altiparanae) exposed to diesel: Evidence of metabolism by aldehyde dehydrogenase in the liver and excretion in water. Ecotoxicology and Environmental Safety, 190, 110, 2020. https://doi.org/10.1016/j.ecoe... PMid:31901814.
28.
KRUGER N.J. The Bradford method for protein quantification. The protein protocols handbook, 17, 2009. https://doi.org/10.1007/978-1-....
29.
MOUNEYRAC C., BUFFET P.E., POIRIER L., ZALOUK-VERGNOUX A., GUIBBOLINI M., RISSO-DE FAVERNEY C., PERREIN-ETTAJNI H. Fate and effects of metal-based nanoparticles in two marine invertebrates, the bivalve mollusc Scrobicularia plana and the annelid polychaete Hediste diversicolor. Environmental Science and Pollution Research, 21 (13), 7899, 2014. https://doi.org/10.1007/s11356... PMid:24647584.
30.
KASEMETS K., IVASK A., DUBOURGUIER H.C., KAHRU A. Toxicity of nanoparticles of ZnO, CuO and TiO2 to yeast Saccharomyces cerevisiae. Toxicology in Vitro, 23, 1116, 2009. https://doi.org/10.1016/j.tiv.... PMid:19486936.
31.
ADAMS L.K., LYON D.Y., ALVAREZ P.J.J. Comparative eco-toxicity of nanoscale TiO2, SiO2, and ZnO water suspensions. Water Research, 40 (19), 3527, 2006. https://doi.org/10.1016/j.watr... PMid:17011015.
32.
ZHENG S., WANG Y., ZHOU Q., CHEN C. Responses of Oxidative Stress Biomarkers and DNA Damage on a Freshwater Snail (Bellamyaaeruginosa) stressed by Ethylbenzene. Archives of environmental contamination and toxicology, 65, 251, 2013. https://doi.org/10.1007/s00244... PMid:23568747.
33.
OTHMANI H. Toxicity evaluation of some heavy metals on the behavior of an animal model from the Coalomates group. Doctoral thesis, University of Annaba, Algeria, 2018.
34.
BESNACI S., BENSOLTANE S., DJEKOUN M. Oxidative stress and histopatological changes induced by the nno-Fe2O3 in Helix aspersa. Scientific Study & Research, 20 (2), 119, 2019.
35.
WUSU A.D., OGUNRINOLA O.O., OJEKALE A.B., OLAITAN S., ONWORDI C.T., AUTA R. The interplay of antioxidant activities in rats exposed to sub chronic low level inorganic mercury is gender specific. Journal of Computer Engineering, 18 (4), 2016.
36.
EGIEBOR E., TULU A., ABOU-ZEID N., OSEJI O.F., ISHAQUE A.B. Oxidative Stress Pathway Mechanisms Induced by Four Individual Heavy Metals (As, Hg, Cd and Pb) and Their Quaternary on MCF-7 Breast Cancer Cells. Journal of Advances in Medicine and Medical Research, 17 (7), 1, 2016. https://doi.org/10.9734/BJMMR/....
37.
EMBABY E., MARGHANI B., ELGHAREEB M., ELMETWALLY M., ABU-HEAKAL N. Protective effect of L-arginine against renal damage induced by cadmium and lead intoxication in rats. Kafrelsheikh Veterinary Medical Journal, 18 (1), 1, 2020. https://doi.org/10.21608/kvmj.....
38.
GASMI A., NOOR S., PISCOPO S., MENZEL A. Toxic Metal -Mediated Neurodegradation: A Focus on Glutathione and GST Gene Variants. Archives of Razi Institute, 77 (2), 525, 2022.
39.
NOWICKA B. Heavy metal-induced stress in eukaryotic algae-mechanisms of heavy metal toxicity and tolerance with particular emphasis on oxidative stress in exposed cells and the role of antioxidant response. Environmental Science and Pollution Research, (12), 16860, 2022. https://doi.org/10.1007/s11356... PMid:35006558 PMCid:PMC8873139.
40.
KOVAČ V., BERGANT M., ŠČANČAR J., PRIMOŽIČ J., JAMNIK P., POLJŠAK B. Causation of Oxidative Stress and Defense Response of a Yeast Cell Model after Treatment with Orthodontic Alloys Consisting of Metal Ions. Antioxidants, 11 (1), 63, 2022. https://doi.org/10.3390/antiox... PMid:35052565 PMCid:PMC8772795.
41.
TASO O.V., PHILIPPOU A., MOUSTOGIANNIS A., ZEVOLIS E., KOUTSILIERIS M. Lipid peroxidation products and their role in neurodegenerative diseases. Annals of research hospitals, 3, 2, 2019. https://doi.org/10.21037/arh.2....
42.
MAS-BARGUES C., ESCRIVÁ C., DROMANT M., BORRÁS C., VIÑA J. Lipid peroxidation as measured by chromatographic determination of malondialdehyde. Human plasma reference values in health and disease. Archives of Biochemistry and Biophysics, 709, 108941, 2021. https://doi.org/10.1016/j.abb.... PMid:34097903.
43.
PAMPANIN D.M., CAMUS L., GOMIERO A., MARANGON I., VOLPATO E., NASCI C. Susceptibility to oxidative stress of mussels (Mytillus galloprovincialis) in Venice Lagon (Italy). Marine Pollution Bulletin, 1548, 2015. https://doi.org/10.1016/j.marp... PMid:16040055.
44.
WANG B., WANG Y., ZHANG J., HU C., JIANG J., LI Y., PENG Z. ROS-induced lipid peroxidation modulates cell death outcome: mechanisms behind apoptosis, autophagy, and ferroptosis. Archives of toxicology, 97, 1439, 2023. https://doi.org/10.1007/s00204... PMid:37127681.
45.
DJEKOUN M., BERREBAH H., DJEBAR M.R. In vivo Cytotoxicity Assessment of Thiram: Physiological and Biochemical Changes in Paramecium sp. Science and technology, 320, 2015.
46.
ASSALY R. Protection du myocarde ischémique et pore géant mitochondrial: applications pharmacologiques, Thèse de Doctorat, Université Paris SUD11, France, 2012.
47.
KOKKINOPOULOU I., MOUTSATSOU P. Mitochondrial Glucocorticoid Receptors and Their Actions. International Journal of Molecular Sciences, 22, 6054, 2021. https://doi.org/10.3390/ijms22... PMid:34205227 PMCid:PMC8200016.
48.
MARZIAZ M.L., FRAZIER K., GUIDRY P.B., RUIZ R.A., PETRIKOVICS I., HAINES D.C. Comparison of brain mitochondrial cytochrome c oxidase activity with cyanide LD(50) yields insight into the efficacy of prophylactics. Journal of Applied Toxicology, 33 (1), 5, 2013. https://doi.org/10.1002/jat.17... PMid:21751223.
49.
ZUHRA K., SZABO C. The two faces of cyanide: an environmental toxin and a potential novel mammalian gasotransmitter. The FEBS Journal, 289 (9), 2481, 2022. https://doi.org/10.1111/febs.1... PMid:34297873 PMCid:PMC9291117.
50.
AL-SHORBAGY Y.M., WADIE W., EL-TANBOULY D.M. Trimetazidine Modulates Mitochondrial Redox Status and Disrupted Glutamate Homeostasis in a Rat Model of Epilepsy. Frontiers in Pharmacology, 12, 735165, 2022. https://doi.org/10.3389/fphar.... PMid:34690772 PMCid:PMC8531497.
51.
DEFFIEU M., BHATIA-KISSOVA I., SALIN B., KLIONSKY B.J., PINSON B., MANON S., CAMOUGRAN N. Increased levels of reduced cytochrome b and mitophagy components are required to trigger nonspecific autophagy following induced mitochondrial dysfunction. Journal of Cell Science, 126, 415, 2012. https://doi.org/10.1242/jcs.10... PMid:23230142 PMCid:PMC4074285.
52.
ZHOU L., ZHAO X., LI M., LU Y., AI C., JIANG C., LIU Y., PAN Z., SHI J. Antifungal activity of silver nanoparticles synthesized by iturin against Candida albicans in vitro and in vivo. Applied Microbiology and Biotechnology, 105 (9), 3759, 2021. https://doi.org/10.1007/s00253... PMid:33900424.
53.
HUSNA, HUSSAIN A., SHAH M., HAMAYUN M., QADIR M., IQBAL A. Heavy metal tolerant endophytic fungi Aspergillus welwitschiae improves growth, ceasing metal uptake and strengthening antioxidant system in Glycine max L. Environmental Science and Pollution Research, (11), 15501, 2022. https://doi.org/10.1007/s11356... PMid:34625902.
54.
AHAMAD I., BANO F., ANWER R., SRIVASTAVA P., KUMAR R., FATMA T. Antibiofilm Activities of Biogenic Silver Nanoparticles Against Candida albicans. Frontiers in Microbiology, 12, 741493, 2022. https://doi.org/10.3389/fmicb.... PMid:35069463 PMCid:PMC8782275.
55.
RYDSTRÖM LUNDIN C., BRZEZINSKI P. Modulation of O2 reduction in Saccharomyces cerevisiae mitochondria. FEBS Letters, 591 (24), 4049, 2017. https://doi.org/10.1002/1873-3... PMid:29171870.
56.
KARRA A.G., SIOUTOPOULOU A., GORGOGIETAS V., SAMIOTAKI M., PANAYOTOU G., PSARRA A.G. Proteomic analysis of the mitochondrial glucocorticoid receptor interacting proteins reveals pyruvate dehydrogenase and mitochondrial 60 kDa heat shock protein as potent binding partners. Journal of Proteomics, 257, 104509, 2022. https://doi.org/10.1016/j.jpro... PMid:35124280.
Share
RELATED ARTICLE
| eISSN: | 2083-5906 |
| ISSN: | 1230-1485 |
We process personal data collected when visiting the website. The function of obtaining information about users and their behavior is carried out by voluntarily entered information in forms and saving cookies in end devices. Data, including cookies, are used to provide services, improve the user experience and to analyze the traffic in accordance with the Privacy policy. Data are also collected and processed by Google Analytics tool (more).
You can change cookies settings in your browser. Restricted use of cookies in the browser configuration may affect some functionalities of the website.
You can change cookies settings in your browser. Restricted use of cookies in the browser configuration may affect some functionalities of the website.
