Microbial Community Dynamics in Field-Scale Biopile Bioremediation
Ana Maria Tănase1,2, Iulia Chiciudean2, Ioana Mereuţă2, Robertina Ionescu2, Călina Petruţa Cornea1, Tatiana Vassu2, Ileana Stoica2
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1Molecular Biology Laboratory, University of Agronomic Sciences and Veterinary Medicine of Bucharest,
59 Mărăşti Blvd, District 1, 011464, Bucharest, Romania
2Department of Genetics, University of Bucharest,
1-3 Portocalelor Aly, Districs 6, 060101, Bucharest, Romania
Submission date: 2016-04-22
Final revision date: 2016-08-08
Acceptance date: 2016-08-12
Online publication date: 2017-01-31
Publication date: 2017-01-31
Pol. J. Environ. Stud. 2017;26(1):331–336
Understanding the microbial communities responses to biopile procedures is an essential step in the field of microbial ecology and in bioremediation applications. In order to estimate metabolic dynamics at a microbial community level, we used Biolog Ecoplates. Assays showed that the bacterial communities from all samples were capable of utilizing a great variety from the 31 carbon substrates. Shannon’s diversity index values were between 2.711±0.303 in August and 3.262±0.062 in April, recording a general variation of 0.5 that is similar to other studies. Statistical analysis showed a good correlation coeficient for 2-hydroxybenzoic acid, a compound that is metabolized through the nafhthalene degradation pathway. Results showed a metabolic shift of the microbial community determined by temperature and the decrease of aromatic hydrocarbon concentrations. The shift was observed in the dynamics of MPN from 9x106 to 1x109 cell/g of soil. Even if the initial total petroleum hydrocarbon concentration from the biopile matrix was much higher than in other studies (10.25 g/kg), at the end of the evaluation the pollutant content of the biopile matrix had a removal rate between 92-98% for monoaromatic hydrocarbons and 57-75% for poliaromatic hydrocarbons.