ORIGINAL RESEARCH
Peroxidases Produced by New Ligninolytic
Bacillus strains Isolated from Marsh and Grassland
Decolourized Anthraquinone and Azo Dyes
| 1 | SAMRC Microbial Water Quality Monitoring Centre, University of Fort Hare, Alice, South Africa |
| 2 | Applied and Environmental Microbiology Research Group (AEMREG), Department of Biochemistry
and Microbiology, University of Fort Hare, Alice, South Africa |
CORRESPONDING AUTHOR
Ayodeji Falade
University of Fort Hare, South Africa, University of Fort Hare, P. Bag X1314, 5700 Alice, South Africa
University of Fort Hare, South Africa, University of Fort Hare, P. Bag X1314, 5700 Alice, South Africa
Submission date: 2018-04-26
Final revision date: 2018-06-12
Acceptance date: 2018-06-22
Online publication date: 2019-05-01
Publication date: 2019-05-28
Pol. J. Environ. Stud. 2019;28(5):3163–3172
KEYWORDS
TOPICS
ABSTRACT
The biotechnological relevance of ligninolytic organisms remains topical and may remain so in
the foreseeable future. The enzyme battery produced by ligninolytic bacteria, including Bacillus
species, has shown immense industrial significance. Consequently, peroxidases produced by newly
isolated ligninolytic Bacillus strains from the marsh and grassland in Hogsback forest reserve of the
Eastern Cape Province of South Africa were evaluated for decolourization of anthraquinone (Remazol
Brilliant Blue R-RBBR) and azo (Congo Red-CR) dyes. Maximum dye decolourization was observed
with the peroxidase from Bacillus sp. NWODO-3: CR (69.89±2.64 %) and RBBR (72.12±0.38 %). Dye
decolourization readings for peroxidases from the other Bacillus strains were CR: 55.06±5.48 %, RBBR:
70.45±0.0 % (Bacillus sp. MABINYA-1), 42.62±5.55 % and 42.42±4.82 % against CR for Bacillus
sp. MABINYA-2 and Bacillus sp. FALADE-1, respectively. RBBR was less susceptible to the attack
by crude peroxidase produced by Bacillus sp. MABINYA-2 and Bacillus sp. FALADE-1 as the dye
decolourization activities observed were 4.91±0.36 % and 1.19±0.0 %, respectively. These results suggest
the industrial relevance of peroxidases from the new ligninolytic Bacillus strains in bioremediation.
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