Peroxidases Produced by New Ligninolytic Bacillus strains Isolated from Marsh and Grassland Decolourized Anthraquinone and Azo Dyes
Ayodeji Falade 1, 2  
,   Leonard Mabinya 1, 2  
,   Anthony Okoh 1, 2  
,   Uchechukwu Nwodo 1, 2  
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SAMRC Microbial Water Quality Monitoring Centre, University of Fort Hare, Alice, South Africa
Applied and Environmental Microbiology Research Group (AEMREG), Department of Biochemistry and Microbiology, University of Fort Hare, Alice, South Africa
Ayodeji Falade   

University of Fort Hare, South Africa, University of Fort Hare, P. Bag X1314, 5700 Alice, South Africa
Submission date: 2018-04-26
Final revision date: 2018-06-12
Acceptance date: 2018-06-22
Online publication date: 2019-05-01
Publication date: 2019-05-28
Pol. J. Environ. Stud. 2019;28(5):3163–3172
The biotechnological relevance of ligninolytic organisms remains topical and may remain so in the foreseeable future. The enzyme battery produced by ligninolytic bacteria, including Bacillus species, has shown immense industrial significance. Consequently, peroxidases produced by newly isolated ligninolytic Bacillus strains from the marsh and grassland in Hogsback forest reserve of the Eastern Cape Province of South Africa were evaluated for decolourization of anthraquinone (Remazol Brilliant Blue R-RBBR) and azo (Congo Red-CR) dyes. Maximum dye decolourization was observed with the peroxidase from Bacillus sp. NWODO-3: CR (69.89±2.64 %) and RBBR (72.12±0.38 %). Dye decolourization readings for peroxidases from the other Bacillus strains were CR: 55.06±5.48 %, RBBR: 70.45±0.0 % (Bacillus sp. MABINYA-1), 42.62±5.55 % and 42.42±4.82 % against CR for Bacillus sp. MABINYA-2 and Bacillus sp. FALADE-1, respectively. RBBR was less susceptible to the attack by crude peroxidase produced by Bacillus sp. MABINYA-2 and Bacillus sp. FALADE-1 as the dye decolourization activities observed were 4.91±0.36 % and 1.19±0.0 %, respectively. These results suggest the industrial relevance of peroxidases from the new ligninolytic Bacillus strains in bioremediation.