Salix matsudana Koidz Tolerance Mechanisms to Cadmium: Uptake and Accumulation, Subcellular Distribution, and Chemical Forms
Hangfeng Wu, Jiayue Wang, Binbin Li, Yangjie Ou, Junran Wang, Qiuyue Shi, Wusheng Jiang, Donghua Liu, Jinhua Zou
More details
Hide details
Tianjin Key Laboratory of Animal and Plant Resistance, College of Life Sciences, Tianjin Normal University,
Tianjin 300387, People’s Republic of China
Submission date: 2016-03-05
Final revision date: 2016-04-17
Acceptance date: 2016-04-18
Publication date: 2016-07-22
Pol. J. Environ. Stud. 2016;25(4):1739–1747
Salix matsudana roots exposed to 10, 50, and 100 μM Cd solutions for 24 h were carried out in order to understand the mechanisms involved in Cd tolerance and detoxification. 50 and 100 μM Cd inhibited root length significantly (P < 0.05). Cd levels in roots increased significantly with increasing Cd concentrations, and the contents of Fe, Mn, Zn, and Ca decreased significantly. A Cd-specific Leadmium Green AM dye probe showed that the meristem zone was the absorption and accumulation site of Cd in the roots. Subcellular fractionation of Cd-containing tissues indicated that about 53% of the Cd was accumulated in the cell wall of S. matsudana roots at 10 μM Cd and 65% of the Cd at 100 μM Cd, indicating that Cd binding and/or precipitation in the cell wall in roots may serve as the first barrier to reduce the cytosolic-free Cd ions. The proportion of CdE and Cdw in roots is low when compared with the other Cd chemical forms. CdHCl, Cdr, and CdHAc represent 46% (10 μM Cd), 49% (50 μM Cd), and 59% (100 μM Cd) of total Cd, and CdNaCl represents 42% (10 μM Cd), 44% (50 μM Cd), and 32% (100 μM Cd).