ORIGINAL RESEARCH
Salix matsudana Koidz Tolerance Mechanisms to Cadmium: Uptake and Accumulation, Subcellular Distribution, and Chemical Forms
Hangfeng Wu, Jiayue Wang, Binbin Li, Yangjie Ou, Junran Wang, Qiuyue Shi, Wusheng Jiang, Donghua Liu, Jinhua Zou
 
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Tianjin Key Laboratory of Animal and Plant Resistance, College of Life Sciences, Tianjin Normal University,
Tianjin 300387, People’s Republic of China
Publish date: 2016-07-22
Submission date: 2016-03-05
Final revision date: 2016-04-17
Acceptance date: 2016-04-18
 
Pol. J. Environ. Stud. 2016;25(4):1739–1747
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ABSTRACT
Salix matsudana roots exposed to 10, 50, and 100 μM Cd solutions for 24 h were carried out in order to understand the mechanisms involved in Cd tolerance and detoxification. 50 and 100 μM Cd inhibited root length significantly (P < 0.05). Cd levels in roots increased significantly with increasing Cd concentrations, and the contents of Fe, Mn, Zn, and Ca decreased significantly. A Cd-specific Leadmium Green AM dye probe showed that the meristem zone was the absorption and accumulation site of Cd in the roots. Subcellular fractionation of Cd-containing tissues indicated that about 53% of the Cd was accumulated in the cell wall of S. matsudana roots at 10 μM Cd and 65% of the Cd at 100 μM Cd, indicating that Cd binding and/or precipitation in the cell wall in roots may serve as the first barrier to reduce the cytosolic-free Cd ions. The proportion of CdE and Cdw in roots is low when compared with the other Cd chemical forms. CdHCl, Cdr, and CdHAc represent 46% (10 μM Cd), 49% (50 μM Cd), and 59% (100 μM Cd) of total Cd, and CdNaCl represents 42% (10 μM Cd), 44% (50 μM Cd), and 32% (100 μM Cd).
eISSN:2083-5906
ISSN:1230-1485