The aim of our study was to estimate the influence of mineral fertilization on the contents of various copper and nickel forms in soil. It was based upon a field experiment made up of ten plots. In average soil samples taken in 2002-04, the pseudo-total copper and nickel content was determined. Chemical forms of these metals, by modified BCR method, were also determined. The nickel content in particular fractions can be arranged quantitatively (average values) in order as follows: F2 (21%) > F3 (18%) > F1 (8%), in the case of copper: F2 (37%)>F3 (14%)>F1 (11%). The accumulation of nickel in the soil during the experiment was not observed. Whereas slight copper accumulation in some plots (with Polifoska 6, Polimag 305, calcium sulfate tetraurea and phosphogypsum) was noted. During the experiment the most mobile nickel and copper forms (soluble + exchangeable fraction) increased.