Plant tissue culture techniques are a valuable tool to obtain suitable plant for phytoremediation and degraded areas because of heavy metals. In this study, the effects of different agar concentrations and different pH levels on in vitro germination parameters studied. In addition, the effects of various concentrations of zinc on growth parameters (root-shoot length, leaf number and fresh weight) and zinc accumulation for Verbascum phrygium endemic plant species investigated. During the in vitro germination period, the best development occurred at 0.7% agar and pH 6.5. In this research, zinc treatment (0, 5, 10, 30, 50, 100 and 250 Zn) was applied for seven days. The mean relative root length, shoot length and leaf number of V. phrygium increased up to 30 mg/L zinc concentration. However fresh weight did not show any significant differences in 0 to 50 mg/L Zn, but they decreased significantly in 100 mg/L Zn. Thus, V. phrygium exhibited high tolerance to increased Zn concentrations. Phytoremediation results indicated that, maximum zinc accumulations were obtained in 10 and 30 mg/L Zn, respectively (18,788.82 mg/kg DW, 18,325.33 mg/kg DW). Although Zn content in the roots and leaves increased with increasing Zn concentration, Zn predominantly accumulated in V. phrygium roots. In conclusion, V. phrygium can be used in contaminated areas due to its capacity to accumulate zinc in its organs.