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eISSN: 2083-5906
ISSN: 1230-1485
ISSN: 1230-1485
ORIGINAL RESEARCH
Biodegradation and Bio-Electricity Generation
of Diesel Oil-Polluted Seawater Via
Laccase-Producing Bacterial Consortium
1
The Institute of Marine Science, Burapha University, Chon Buri 20131, Thailand
2
Department of Pharmaceutical Chemistry and Pharmacognosy, Faculty of Pharmaceutical Science,
Naresuan University, Phitsanulok 65000, Thailand
3
Department of Mechanical Engineering, Faculty of Engineering, Princess of Naradhiwas University,
Naradhiwas 96000, Thailand
4
Department of Biological Science, Faculty of Science and Digital Innovation, Thaksin University,
Phatthalung 93210, Thailand
Submission date: 2025-05-01
Final revision date: 2025-07-21
Acceptance date: 2025-08-10
Online publication date: 2025-10-15
Corresponding author
Pimprapa Chaijak
Department of Biological Science, Faculty of Science and Digital Innovation, Thaksin University, Phatthalung 93210, Thailand
Department of Biological Science, Faculty of Science and Digital Innovation, Thaksin University, Phatthalung 93210, Thailand
KEYWORDS
TOPICS
ABSTRACT
Petroleum contamination is a significant concern for both the environment and human health.
Various methods have been used for the remediation of petroleum-contaminated seawater. In this study,
a laccase-producing bacterial consortium was selected and used for the remediation of dieselcontaminated
seawater. Moreover, this consortium was integrated with a microbial fuel cell to recover
electrical energy from the diesel degradation. The results found that the bacterial consortium MS
exhibited the highest laccase activity (28.13±0.20 U/mL) and achieved a 97.85±0.52% diesel degradation
rate. Metabolomic analysis revealed the presence of several degradation products, including ethylbenzene,
1,3-dimethylbenzene, propylbenzene, 1-(1-propynyl)-1-cyclohexene, 1,2,4-trimethylbenzene, benzene,
1,1’-(1,2-dimethyl-1,2-ethanediyl) bis, and hentriacontane. Furthermore, a floating microbial fuel cell
(MFC) coupled with the consortium generated a maximum power density (PD) of 0.18±0.00 W/m3
and a maximum current density (CD) of 0.53±0.01 A/m3. These findings highlight the potential of
marine bacterial consortia with laccase activity for bioremediation of diesel-contaminated seawater,
coupled with the added benefit of bioenergy recovery.
CONFLICT OF INTEREST
The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
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| eISSN: | 2083-5906 |
| ISSN: | 1230-1485 |
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