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eISSN: 2083-5906
ISSN: 1230-1485
ISSN: 1230-1485
ORIGINAL RESEARCH
Characterization of Antibacterial Compounds
from Seaweed Against Pathogenic Bacteria
1
Marine Science, Faculty of Fisheries and Marine Science, Diponegoro University, Central Java, 50275, Indonesia
2
Department of Biology, Faculty of Biology, Jenderal Soedirman University. Jl. dr. Suparno 63 Grendeng,
Purwokerto 53122, Central Java, Indonesia
3
Research Center for Oceanography, National Research and Innovation Agency (BRIN), Jakarta,14430, Indonesia
Submission date: 2024-01-16
Final revision date: 2024-05-20
Acceptance date: 2024-06-05
Online publication date: 2024-09-09
Publication date: 2025-05-09
Corresponding author
Wilis Ari Setyati
Department of Marine Science, Faculty of Fisheries and Marine Science, Diponegoro University, Jl. Prof. H. Soedarto, S.H. No.1. Tembalang, Semarang, Central Java, 50275, Indonesia, Indonesia
Department of Marine Science, Faculty of Fisheries and Marine Science, Diponegoro University, Jl. Prof. H. Soedarto, S.H. No.1. Tembalang, Semarang, Central Java, 50275, Indonesia, Indonesia
Pol. J. Environ. Stud. 2025;34(4):4255-4261
KEYWORDS
TOPICS
ABSTRACT
Bacterial communities collaborating with seaweed play an important role in many aspects, especially
in the production of secondary metabolites that help ecosystem function and have good prospects for pharmacological
utilization. The objective of this study was to determine the antibacterial activity of associated bacteria
isolated from seaweed in Awur Bay, Jepara, Indonesia. The antibacterial test uses the pathogenic
bacteria Staphylococcus aureus, Pseudomonas aeruginosa, and Micrococcus luteus. Isolation of
seaweed-associated bacteria obtained 4 potential isolates, which were closely related to Vibrionaceae
bacterium PH25 (99.86%), Vibrio alginolyticus strain GS MYPK1 (99.65%), Salinivibrio costicola
strain M318 (99.86%), and Vibrio alginolyticus strain 2014V-1011 (99.93%). In disc diffusion, MIC,
and MBC tests, four isolates showed potential antibacterial activity. TLC identified groups of compounds
that were reported to have antibacterial activity, such as alkaloids, terpenoids, steroids, and flavonoids. Compounds
that were reported to have antibacterial properties were identified from GCMS, one of which was
the compound azulane (CAS).
CONFLICT OF INTEREST
The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
REFERENCES (42)
1.
EGAN S., HARDER T., BURKE C., STEINBERG P., KJELLEBERG S., THOMAS T. The seaweed holobiont: Understanding seaweed-bacteria interactions. FEMS Microbiology Reviews, 37, 462, 2013. https://doi.org/10.1111/1574-6... PMid:23157386.
2.
JUHMANI A.S., VEZZI A., WAHSHA M., BUOSI A., PASCALE F.D.E., SCHIAVON R., SFRISO A. Diversity and dynamics of seaweed associated microbial communities inhabiting the lagoon of Venice. Microorganisms, 8 (11), 1, 2020. https://doi.org/10.3390/microo... PMid:33114532 PMCid:PMC7693704.
3.
SUSANTO A.B., SANTOSA G.W., DJUNAEDI A., PRINGGENIES D., ARIYANTO D. The impact of mixed feed of seaweed Gracilaria sp. and transfer factor formula on the immune response of Nile tilapia (Oreochromis niloticus). AACL Bioflux, 16 (1), 242, 2023.
4.
MADIBANA M.J., MLAMBO V., LEWIS B., FOUCHÉ C. Effect of graded levels of dietary seaweed (Ulva sp.) on growth, hematological and serum biochemical parameters in dusky kob, Argyrosomus japonicus, sciaenidae. Egyptian Journal of Aquatic Research, 43 (3), 249, 2017. https://doi.org/10.1016/j.ejar....
5.
PRINGGENIES D., RETNOWATI E.I., ARIYANTO D., DEWI K., VIHARYO M.A.S., SUSILOWATI R. Symbiotic microbes from various seaweeds with antimicrobial and fermentative properties. AACL Bioflux, 13 (4), 2211, 2020.
6.
MENAA F., WIJESINGHE P.A.U.I., THIRIPURANATHAR G., UZAIR B., IQBAL H., KHAN B.A., MENAA B. Ecological and industrial implications of dynamic seaweed-associated microbiota interactions. Marine Drugs, 18 (12), 1, 2020. https://doi.org/10.3390/md1812... PMid:33327517 PMCid:PMC7764995.
7.
PAMUNGKAS D.B.P., SETYATI W.A., RYANDINI D. Antibacterial ability of seaweed endophytic bacteria (Turbinaria ornata, Sargassum crassifolium, and Sargassum polycystum) against skin disease agents. Trends in Sciences, 21 (2), 7282-7282, 2024. https://doi.org/10.48048/tis.2....
8.
SANTOSA G.W., DJUNAEDI A., SUSANTO A., PRINGGENIES D., ARIYANTO D., PRANOTON A.K. The potential two types of green macroalgae (Caulerpa racemosa and Caulerpa lentillifera) as a natural food preservative from Jepara beach, Indonesia. Trends in Sciences, 21 (5), 739ss4, 2024. https://doi.org/10.48048/tis.2....
9.
SKOWRON K., BAUZA-KASZEWSKA J., KRASZEWSKA Z., WIKTORCZYK-KAPISCHKE N., GRUDLEWSKA-BUDA K., KWIECIŃSKA-PIRÓG J., WAŁECKA-ZACHARSKA E., RADTKE L., GOSPODAREK-KOMKOWSKA E. Human skin microbiome: impact of intrinsic and extrinsic factors on skin microbiota. Microorganisms, 9, 543, 2021. https://doi.org/10.3390/microo... PMid:33808031 PMCid:PMC7998121.
10.
CARMONA-CRUZ S., OROZCO-COVARRUBIAS L., SÁEZ-DE-OCARIZ M. The human skin microbiome in selected cutaneous diseases. Frontiers in Cellular and Infection Microbiology, 12, 834135, 2022. https://doi.org/10.3389/fcimb.... PMid:35321316 PMCid:PMC8936186.
11.
SUN L., LIU W., ZHANG L.J. The role of toll-like receptors in skin host defense, psoriasis, and atopic dermatitis. Journal of Immunology Research, 2019, 1824624, 2019. https://doi.org/10.1155/2019/1... PMid:31815151 PMCid:PMC6877906.
12.
BYRD A.L., BELKAID Y., SEGRE J.A. The human skin microbiome. Nature Reviews Microbiology, 16 (3), 143, 2018. https://doi.org/10.1038/nrmicr... PMid:29332945 PMCid:PMC12237257.
13.
PARLET C.P., BROWN M.M., HORSWILL A.R. Commensal Staphylococci influence Staphylococcus aureus skin colonization and disease. Trends Microbiology, 27 (6), 497, 2019. https://doi.org/10.1016/j.tim.... PMid:30846311 PMCid:PMC7176043.
14.
ELAHI M.G., HEKMATI M., ESMAEILI D., ZIARATI P., YOUSEFI M. Antibacterial activity of modified carvacrol against Staphylococcus epidermidis and Pseudomonas aeruginosa. Novelty in Clinical Medicine, 1 (4), 192, 2022.
15.
GUPTA V., CHAUHAN A., KUMAR R.N.S., DHYANI A., CHAKRAVARTY S. Meningitis caused by micrococcus luteus: Case report and review of literature. IP International Journal of Medical Microbiology and Tropical Diseases, 5 (1), 63, 2019. https://doi.org/10.18231/2581-....
16.
SINGH R.P., REDDY C.R.K. Seaweed-microbial interactions: key functions of seaweed-associated bacteria. FEMS Microbiology Ecology, 88 (2), 213, 2014. https://doi.org/10.1111/1574-6... PMid:24512602.
17.
HOLLANTS J., LELIAERT F., DE CLERCK O., WILLEMS A. What we can learn from sushi: A review on seaweed-bacterial associations. FEMS Microbiology Ecology, 83 (1), 1, 2013. https://doi.org/10.1111/j.1574... PMid:22775757.
18.
SETYATI W.A., SUSANTO A.B., PAMUNGKAS D.B.P., MAKRIMA D.B., SENDUK J.L. Isolation and identification of seaweed-associated bacteria and their antibacterial activity against skin disease agents. Trends in Sciences, 20, 6, 2023. https://doi.org/10.48048/tis.2....
19.
SUJULIYANI., THAIB E.A., INDRIATI N., LIANANDA M. Antibacterial activity of the symbiotic bacteria of green algae Caulerpa racemosa from Pulau Lima Indonesia. IOP Conf. Series: Earth and Environmental Science, 012075, 1, 2019. https://doi.org/10.1088/1755-1....
20.
KOWALSKA-KROCHMAL B., DUDEK-WICHER R. The minimum inhibitory concentration of antibiotics: methods, interpretation, clinical relevance. Pathogens, 10, 165, 2021. https://doi.org/10.3390/pathog... PMid:33557078 PMCid:PMC7913839.
21.
AL-KAFAWEEN M.A., HILMI A.B.M., JAFFAR N., ALJAMAL H.A.N., ZAHRI M.K., JIBRIL F.I. Antibacterial and antibiofilm activities of Malaysian Trigona honey against Pseudomonas aeruginosa ATCC 10145 and Streptococcus pyogenes ATCC 19615. Jordan Journal of Biological Sciences, 13 (1), 69, 2020.
22.
BELE A.A., KHALE A. An Overview on Thin Chromatography. Journal of Pharmaceutical Sciences, 2 (2), 256, 2011.
23.
DIAS M.C., PINTO D.C.G.A., SILVA A.M.S. Plant flavonoids: Chemical characteristics and biological activity. Molecules, 26, 5377, 2021. https://doi.org/10.3390/molecu... PMid:34500810 PMCid:PMC8434187.
24.
IQBAL E., SALIM K.A., LIM L.B.L. Phytochemical screening, total phenolics and antioxidant activities of bark and leaf extracts of Goniothalamus velutinus (Airy Shaw) from Brunei Darussalam. Journal of King Saud University - Science, 27 (3), 224, 2015. https://doi.org/10.1016/j.jksu....
25.
PATI S., TUFARIELLO M., CRUPI P., COLETTA A., GRIECO F., LOSITO I. Quantification of volatile compounds in wines by HS-SPME-GC/MS: Critical issues and use of multivariate statistics in method optimization. Processes, 9 (662), 1, 2021. https://doi.org/10.3390/pr9040....
26.
JALEEL W., LI Q., SHI Q., QI G., LATIF M., ALI S., HE Y. Using GCMS to find out the volatile components in the aroma of three different commercial fruits in China. JAPS: Journal of Animal & Plant Sciences, 31 (1), 2021. https://doi.org/10.36899/JAPS.....
27.
RODRÍGUEZ-MELCÓN C., ALONSO-CALLEJA C., GARC C., CARBALLO J., CAPITA R. Bactericidal concentration (MBC) for twelve antimicrobials. Biology, 11, 46, 2022. https://doi.org/10.3390/biolog... PMid:35053044 PMCid:PMC8773323.
28.
HU Y., CHEN S., YANG F., DONG S. Marine Indole Alkaloids - Isolation, Structure and Bioactivities. Marine Drugs, 19, 658, 2021. https://doi.org/10.3390/md1912... PMid:34940657 PMCid:PMC8708922.
29.
OTHMAN L., SLEIMAN A., ABDEL-MASSIH R.M. Antimicrobial activity of polyphenols and alkaloids in middle eastern plants. Frontiers in Microbiology, 10, 1, 2019. https://doi.org/10.3389/fmicb.... PMid:31156565 PMCid:PMC6529554.
30.
ALMEIDA M.C., RESENDE D.I.S.P., DA COSTA P.M., PINTO M.M.M., SOUSA E. Tryptophan derived natural marine alkaloids and synthetic derivatives as promising antimicrobial agents. European Journal of Medicinal Chemistry, 209, 112945, 2021. https://doi.org/10.1016/j.ejme... PMid:33153766.
31.
YAN Y., LI X., ZHANG C.L.V.L., GAO B., LI M. Research progress on antibacterial activities and mechanisms of natural alkaloids: A review. Antibiotics, 10, 318, 2021. https://doi.org/10.3390/antibi... PMid:33808601 PMCid:PMC8003525.
32.
GOZARI M., ALBORZ M., EL-SEEDI H.R., JASSBI A.R. Chemistry, biosynthesis and biological activity of terpenoids and meroterpenoids in bacteria and fungi isolated from different marine habitats. European Journal of Medicinal Chemistry, 210, 112957, 2021. https://doi.org/10.1016/j.ejme... PMid:33160760.
33.
HUANG W., WANG Y., TIAN W., CUI X., TU P., LI J., SHI S., LIU X. Flavonoid Antimicrobial Agents Derived from Medicinal Plants. Journal Antibiotics, 11 (1380), 1, 2022. https://doi.org/10.3390/antibi... PMid:36290037 PMCid:PMC9598646.
34.
MARTINS B.T., DA SILVA M.C., PINTO M., CIDADE H., KIJJOA A. Marine natural flavonoids: chemistry and biological activities. Natural Product Research, 33, 3260, 2019. https://doi.org/10.1080/147864... PMid:29726719.
35.
DONADIO G., MENSITIERI F., SANTORO V., PARISI V., BELLONE M.L., DE TOMMASI N., IZZO V., DAL PIAZ F. Interactions with microbial proteins driving the antibacterial activity of flavonoids. Pharmaceutics, 13, 660, 2021. https://doi.org/10.3390/pharma... PMid:34062983 PMCid:PMC8147964.
36.
PADMAVATHI A.R., ABINAYA B., PANDIAN S.K. Phenol, 2,4-bis(1,1-dimethylethyl) of marine bacterial origin inhibits quorum sensing mediated biofilm formation in the uropathogen Serratia marcescens. Journal of Bioadhesion and Biofilm Research, 30, 1111, 2014. https://doi.org/10.1080/089270... PMid:25377484.
37.
LEYLAIE S., ZAFARI D. Antiproliferative and antimicrobial activities of secondary metabolites and phylogenetic study of endophytic Trichoderma species From Vinca Plants. Frontiers in Microbiology, 9, 1, 2018. https://doi.org/10.3389/fmicb.... PMid:30050508 PMCid:PMC6051055.
38.
LAMMERS A., ZWEERS H., SANDFELD T., BILDE T., GARBEVA P., SCHRAMM A., LALK M. Antimicrobial compounds in the volatilome of social spider communities. Frontiers in Microbiology, 12, 700693, 2021. https://doi.org/10.3389/fmicb.... PMid:34504476 PMCid:PMC8422909.
39.
ANDILA P.S., NUGROHO L.H. Antibacterial and phytochemical constituent of Etlingera rubroloba A.D. Poulsen extract, an endemic ginger from Wallacea Region, Indonesia. Biodiversitas, 23, 3646, 2022. https://doi.org/10.13057/biodi....
40.
KRISHNAMOORTHY K., SUBRAMANIAM P. Phytochemical profiling of leaf, stem, and tuber parts of Solena amplexicaulis (Lam.) Gandhi using GC-MS. International Scholarly Research Notices, 2014, 1, 2014. https://doi.org/10.1155/2014/5... PMid:27379314 PMCid:PMC4897340.
41.
SHAABAN M.T., GHALY M.F., FAHMI S.M. Antibacterial activities of hexadecanoic acid methyl ester and green-synthesized silver nanoparticles against multidrug-resistant bacteria. Journal of Basic Microbiology, 61, 557, 2021. https://doi.org/10.1002/jobm.2... PMid:33871873.
42.
ALSUFYANI T., AL-OTAIBI N., ALOTAIBI N.J., M’SAKNI N.H., ALGHAMDI E.M. GC Analysis, Anticancer, and antibacterial activities of secondary bioactive compounds from endosymbiotic bacteria of pomegranate aphid and its predator and protector. Molecules, 28, 4255, 2023. https://doi.org/10.3390/molecu... PMid:37241995 PMCid:PMC10222457.
CITATIONS (2):
1.
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2.
Synergistic Effects of Seaweed Inclusion and Gambier Extract as a Natural Feed Additive on Rumen Fermentation and Methane Reduction In Vitro
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Roni Pazla, Asmuddin Natsir, Rahmat Hidayat, Nurzainah Ginting, Antonius Antonius, Zaitul Ikhlas, Leni Marlina, Nurhafsah Nurhafsah, Rosniati Rosniati, Misgiyarta Misgiyarta, Manuel Alcaraz, Hendri Ferianson P. Purba, Rosniyati Suwarda, Sitti Ramlah, Procula Rudlof Matitaputty
| eISSN: | 2083-5906 |
| ISSN: | 1230-1485 |
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