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ORIGINAL RESEARCH
Characterization of Antibacterial Compounds
from Seaweed Against Pathogenic Bacteria
1
Marine Science, Faculty of Fisheries and Marine Science, Diponegoro University, Central Java, 50275, Indonesia
2
Department of Biology, Faculty of Biology, Jenderal Soedirman University. Jl. dr. Suparno 63 Grendeng,
Purwokerto 53122, Central Java, Indonesia
3
Research Center for Oceanography, National Research and Innovation Agency (BRIN), Jakarta,14430, Indonesia
Submission date: 2024-01-16
Final revision date: 2024-05-20
Acceptance date: 2024-06-05
Online publication date: 2024-09-09
Publication date: 2025-05-09
Corresponding author
Wilis Ari Setyati
Department of Marine Science, Faculty of Fisheries and Marine Science, Diponegoro University, Jl. Prof. H. Soedarto, S.H. No.1. Tembalang, Semarang, Central Java, 50275, Indonesia, Indonesia
Department of Marine Science, Faculty of Fisheries and Marine Science, Diponegoro University, Jl. Prof. H. Soedarto, S.H. No.1. Tembalang, Semarang, Central Java, 50275, Indonesia, Indonesia
Pol. J. Environ. Stud. 2025;34(4):4255-4261
KEYWORDS
TOPICS
ABSTRACT
Bacterial communities collaborating with seaweed play an important role in many aspects, especially
in the production of secondary metabolites that help ecosystem function and have good prospects for pharmacological
utilization. The objective of this study was to determine the antibacterial activity of associated bacteria
isolated from seaweed in Awur Bay, Jepara, Indonesia. The antibacterial test uses the pathogenic
bacteria Staphylococcus aureus, Pseudomonas aeruginosa, and Micrococcus luteus. Isolation of
seaweed-associated bacteria obtained 4 potential isolates, which were closely related to Vibrionaceae
bacterium PH25 (99.86%), Vibrio alginolyticus strain GS MYPK1 (99.65%), Salinivibrio costicola
strain M318 (99.86%), and Vibrio alginolyticus strain 2014V-1011 (99.93%). In disc diffusion, MIC,
and MBC tests, four isolates showed potential antibacterial activity. TLC identified groups of compounds
that were reported to have antibacterial activity, such as alkaloids, terpenoids, steroids, and flavonoids. Compounds
that were reported to have antibacterial properties were identified from GCMS, one of which was
the compound azulane (CAS).
CONFLICT OF INTEREST
The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
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| eISSN: | 2083-5906 |
| ISSN: | 1230-1485 |
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