Column switching and liquid chromatographic technique for the rapid determination of fenoxycarb insecticide residues in apples
Michel M, Krause A, Buszewski B
 
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Buszewski B, Nicholas Copernicus Univ, Fac Chem, Dept Environm Chem & Ecoanalyt, 7 Gagarina St, PL-87100 Torun, Poland
Nicholas Copernicus Univ, Fac Chem, Dept Environm Chem & Ecoanalyt, PL-87100 Torun, Poland
Plant Protect Inst Poznan, Field Expt Stn Torun, Pesticide Residue Lab, PL-87100 Torun, Poland
 
 
Pol. J. Environ. Stud. 2001;10(4):283-287
 
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ABSTRACT
Fenoxycarb residues are analyzed by column switching and reversed-phase high performance liquid chromatography (RP-HPLC). The active ingredient is extracted from apples on a silica gel column using a n-hexane - diethyl ether mixture. The eluate is evaporated, dry residue dissolved in acetonitrile-deionized water, and injected into the liquid chromatograph with a column switching system (C8 columns), and a UV-photodiode array detector (UV - PDA). The analyte is quantified by the external standard method. The average recoveries of the active ingredient from the spiked sample are 81.3 +/- 3.2% and 80.3 +/- 5.8%, the coefficients of variation are 3.9% and 7.2% for fortification levels 0.1 mg/kg and 0.05 mg/kg, respectively, and the limit of quantification at lambda = 228 nm is 0.05 mg/kg. Labor and organic solvent uses are greatly reduced in comparison to the existing methods. The overall procedure allows a sample throughput of up to 30 samples per day. The method was applied to the determination of fenoxycarb residue in apples from treated orchards.
CONFLICT OF INTEREST
The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
eISSN:2083-5906
ISSN:1230-1485
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