ORIGINAL RESEARCH
Comparative Efficacy of Temephos
and Pyriproxyfen on Aedes aegypti,
Aedes albopictus, and Culex quinquefasciatus
Collected from Different Ecological
Zones of Punjab, Pakistan
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1
Department of Entomology, Faculty of Agriculture and Environment, The Islamia University of Bahawalpur,
Punjab, Pakistan
2
Department of Entomology, Faculty of Agriculture, University of Agriculture Faisalabad, Punjab, Pakistan
3
Nawab Sir Sadiq Muhammad Khan Abbasi Hospital, Bahawapur 63100, Pakistan
4
Department of Agronomy, The Islamia University of Bahawalpur, Bahawalpur-63100, Pakistan
5
Department of Life Sciences, Western Caspian University, Baku, Azerbaijan
6
Department of Environmental Sciences, Shaheed Benazir Bhutto University Sheringal, Dir (U),
Khyber Pakhtunkhwa 18000 Pakistan
7
School of Biological Sciences, The University of Western Australia, 35 Stirling Highway, Perth, WA 6009, Australia
8
Department of Entomology, Bahauddin Zakariya University Multan, Pakistan
9
College of Science, King Saud University, P.O.Box-2454, Riyadh-11451, Saudi Arabia
10
Department of Zoology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia
Submission date: 2024-02-07
Final revision date: 2024-04-03
Acceptance date: 2024-06-28
Online publication date: 2024-11-13
Publication date: 2025-06-06
Corresponding author
Rashid Iqbal
Department of Agronomy, The Islamia University of Bahawalpur, Bahawalpur-63100, Pakistan
Allah Ditta
Department of Environmental Sciences, Shaheed Benazir Bhutto University Sheringal, Dir (U),
Khyber Pakhtunkhwa 18000 Pakistan
Pol. J. Environ. Stud. 2025;34(4):4577-4586
KEYWORDS
TOPICS
ABSTRACT
Management of mosquitoes accentuates mostly the use of both larvicides and adulticides.
The dengue control program in Punjab, Pakistan, has long been under the influence of temephos
(1G and 50EC) for larviciding. The present study was therefore planned to use larvicides that were
currently being used in a dengue control program along with insect growth regulators (IGR) against
mosquito species (Culex and Aedes) collected from various ecological regions of Punjab (Pakistan)
regarding disease outbreaks to assess their efficiency by performing larval bioassays as suggested by
the World Health Organization (WHO) at three different doses, i.e., low dose (D1), recommended dose
(D2), and high dose (D3). Results revealed that among the three larvicides used, pyriproxyfen 0.5WDG
showed significantly higher control than temephos 1G and temephos 50EC, but in the case of temephos
1G and temephos 50EC, temephos 1G showed slightly better control. A marginal difference was observed between the recommended doses (D2) and the higher doses (D3), but a substantial difference
was observed between the lower doses (D1) and those of the recommended and higher doses (D2
and D3). Pyriproxyfen 0.5WDG at the recommended dose should be applied in mosquito control
programs relying heavily on larvicides as it proved to be a promising control agent, followed
by temephos 1G and temephos 50EC.
CONFLICT OF INTEREST
The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
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