The correlation between road network accessibility and land use is mainly considered a spatial-topological relationship, thus making it difficult to reveal accurately. In consideration of the road speed limit and network structure, this paper constructed a road network accessibility model based on weighted spatial syntax. A correlation analysis between road network accessibility and land use was then conducted and compared with those based on relative asymmetry (RA) and normalized angular choice (NACH) by regarding the central district of Fuzhou City as an example. Results showed that the roads with good accessibility by RA and NACH were presented as ‘grid’ roads. The result of road network accessibility by weighted RA and NACH could better reflect the actual effect of road network accessibility than RA and NACH. Furthermore, the road network accessibility by weighted RA and NACH could improve the correlation analysis between road network accessibility and urban land use, especially in NACH. Results could provide a scientific reference for the urban planning and development.