The study was conducted at the College of Agricultural Engineering, University of Baghdad, for 2021-2022. The morphological and molecular examination results of the fungal isolates causing the disease revealed that they belong to R. solani. The results indicated that the fungal isolate Rn1 was the most pathogenic, reaching a severity rating of 4.3 (severely pathogenic) and achieving a percentage of 6.65% for seed germination compared to the control treatment, which reached 95.3%. The results of the electrophoretic migration of DNA extracted from the fungal isolate showed the presence of a single band with a molecular weight of 550 bp. The nucleotide sequence results showed that the R. solani fungus isolates recorded a 100% match with the global isolates in the NCBI gene bank, deposited with the World Gene Bank under accession number OR497844.1. The results demonstrated the effectiveness of aqueous Calotropis procera extract and the pesticide in inhibiting fungal growth, with inhibitory rates of 64.70% and 65%, respectively, compared to 0% for the control treatment. The results of the field experiment showed that the percentage of the severity of infection reached 8 and 20%, respectively, for the vegetative and root mass by immersion and reached 28 and 48% by dusting compared to the control treatment, which recorded 80%, and 86.67% respectively. The results also indicated the efficacy of Calotropis procera extract with significant differences in productivity, reaching 2116.67 and 1150 grams, respectively, while in the control treatment, it was 616.67 grams.