ORIGINAL RESEARCH
Impact of Polyethylene and Polylactic Acid Microplastics on Seed Germination and Seedling Development of Trifolium repens
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1
School of Karst Science, Guizhou Normal University, Guiyang, Guizhou 550025, China
 
2
School of Life Sciences, Guizhou Normal University, Guiyang, Guizhou 550025, China
 
These authors had equal contribution to this work
 
 
Submission date: 2025-11-07
 
 
Final revision date: 2026-02-24
 
 
Acceptance date: 2026-02-27
 
 
Online publication date: 2026-06-15
 
 
Corresponding author
Yang Gao   

School of Karst Science, Guizhou Normal University, Guiyang, Guizhou 550025, China
 
 
Puchang Wang   

School of Life Sciences, Guizhou Normal University, Guiyang, Guizhou 550025, China
 
 
 
KEYWORDS
TOPICS
ABSTRACT
Microplastics (MPs) are emerging contaminants with uncertain effects on plants, particularly ornamentals. We tested how polyethylene (PE), polylactic acid (PLA), and a constant-total-dose 1:1 (w/w) mixture of PE and PLA (MIX; 25 μm) at 0.1% (w/v), 0.5% (w/v), and 1.0% (w/v) influence seed germination and early growth of white clover (Trifolium repens) in Petri-dish assays. Although these exposure levels exceed typical background levels in bulk soils, they were used to represent worst-case/high-exposure scenarios to elucidate potential mechanisms and effect thresholds in a 7-day assay. Endpoints included germination potential and final germination rate, root traits (radicle elongation inhibition rate and root-to-shoot ratio), seedling biomass (fresh/dry mass), and water content. Responses generally followed a dose-dependent hormetic pattern, with low-dose PE increasing final germination rate, while higher exposures impaired root development and altered biomass allocation. Notably, the high-dose MIX treatment reduced the root-to-shoot ratio by ~37% relative to the control, suggesting disrupted allocation and/or disproportionate root impairment under intense MP stress. Water relations were polymer-specific: PE reduced seedling water content across all tested levels, consistent with physical blockage and/or impaired root water transport, whereas PLA and MIX caused significant declines mainly at higher concentrations. Overall, polymer identity and concentration jointly regulated early establishment, supporting the “low-dose stimulation, high-dose inhibition” framework and underscoring the need to consider both biodegradable and conventional MPs in ecological risk assessment.
CONFLICT OF INTEREST
The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
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