In this study, the methanolic extract of Raphanus sativus (RSME) seeds was evaluated for its protective effect against CCl₄^-induced nephrotoxicity. The treatment of Swiss albino rats with intraperitoneal injections of CCl₄ (1 ml/kg body weight) on alternate days for 30 days decreased the antioxidant enzymes, while lipid peroxidation and serum toxicity markers were increased. These changes were reversed in the animals receiving an oral dose of RSME (100 and 200 mg/kg body weight) along with CCl₄, thus increasing the level of antioxidant enzymes and decreasing serum toxicity markers and thus ameliorating the toxic effect of CCl₄. These results show that seed extract of R. sativus can reciprocate the toxic effects of CCl₄ and can be used to make a chemopreventive drug.