The objective of the study was to determine the effects of habitat conditions on the content of nutrients in the seeds of different cultivars of blue and yellow lupine. Experiments were located in different parts of Poland. Sixteen cultivars of blue lupine and eight cultivars of yellow lupine were used in these experiments. The study showed that yellow lupine seeds had a higher protein content then blue lupine, and the contents of this component were varied between both cultivars of this species depending on examined factors, while the fiber content was little varied between species and cultivars. The average fat content in the seeds of blue lupine was slightly higher, the amount of starch was a much higher, and the water-soluble sugars slightly lower than in the seeds of yellow lupine. Significant differences between cultivars of blue lupine were found in the contents of crude fat, water-soluble carbohydrates and alkaloids, and between cultivars of yellow lupine in the amounts of crude fat. The cultivation region did not significantly affect only starch accumulation by the tested lupine cultivars as well as alkaloids in yellow lupine. The contents of the other components were varied depending on the area of cultivation.