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SHORT COMMUNICATION
Polyunsaturated Fatty Acid (PUFA) Production
by Microalgae Using Palm Oil Mill Effluent
as a Substrate Under Dark Stress
1
Department of Microbiology, Faculty of Science and Digital Innovation, Thaksin University,
Phatthalung 93210, Thailand
2
Thailand Institute of Nuclear Technology (Public Organization) (TINT), Nakhon Nayok 26120, Thailand
3
Department of Biology, Faculty of Science and Digital Innovation, Thaksin University, Phatthalung 93210, Thailand
Submission date: 2023-11-03
Final revision date: 2024-02-22
Acceptance date: 2024-04-06
Online publication date: 2024-07-29
Publication date: 2025-05-09
Corresponding author
Pimprapa Chaijak
Department of Biology, Faculty of Science and Digital Innovation, Thaksin University, Phatthalung 93210, Thailand
Department of Biology, Faculty of Science and Digital Innovation, Thaksin University, Phatthalung 93210, Thailand
Pol. J. Environ. Stud. 2025;34(4):4481-4487
KEYWORDS
TOPICS
ABSTRACT
This study explores the potential of Chlorella sp. BF03 isolated from a betta fish farm is a promising
candidate for sustainable biomass and polyunsaturated fatty acid (PUFA) production when cultivated
in palm oil mill effluent (POME). The investigation reveals substantial improvements in both biomass
quantity and PUFA content under specific cultivation conditions. The most favorable outcomes were
achieved when Chlorella sp. BF03 was grown in POME at a dilution ratio of 1:3, resulting in the
highest biomass yield of 153.03±36.16 mg/L. Gas chromatography-mass spectrometry (GC-MS) was
employed for the analysis of the fatty acid profile. The results revealed the presence of α-linolenic acid
(ALA). To optimize fatty acid production, Chlorella sp. BF03 was subjected to dark stress. The findings
present compelling evidence of enhanced polyunsaturated fatty acid (PUFA) content, emphasizing
the potential of Chlorella sp. BF03 for sustainable PUFA production using palm oil mill effluent
(POME) as a substrate.
CONFLICT OF INTEREST
The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
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