The interaction between soil quality and the potential of Paulownia cultivation in terms of revitalization of a specific agricultural brownfield located in the East Slovak region of the Slovak Republic is studied. At the same time, the qualitative indicators of soil and phytomass, including planted Paulownia seedlings, to further possible revitalization of agricultural brownfields in terms of the obtained soil quality data is presented. Based on the obtained qualitative data on the soil, originally occurring phytomass, summary findings and recommendations for revitalizing a particular agricultural brownfield in Paulownia are presented, as fast-growing trees are supporting not only the revitalization of a specific agricultural brownfield but also the climate change mitigation. At the same time, selected indicators of Paulownia’s growth are used to potentially revitalize the agricultural brownfield with the aim of its practical use.