ORIGINAL RESEARCH
Application of Lignocellulosic Waste Materials
for the Production and Stabilization
of Trichoderma Biomass
Anna Kancelista1, Urszula Tril2, Regina Stempniewicz1, Michał Piegza1,
Magdalena Szczech3, Danuta Witkowska1
1Department of Biotechnology and Food Microbiology,
Wrocław University of Environmental and Life Sciences, Chełmońskiego 37/41, 51-630 Wrocław, Poland
2Department of Animal Products Technology and Quality Management,
Wrocław University of Environmental and Life Sciences, Chełmońskiego 37/41, 51-630 Wrocław, Poland
3Department of Vegetable Plant Protection, Research Institute of Horticulture in Skierniewice,
Konstytucji 3 Maja 1/3, 96-100 Skierniewice, Poland
Wrocław University of Environmental and Life Sciences, Chełmońskiego 37/41, 51-630 Wrocław, Poland
2Department of Animal Products Technology and Quality Management,
Wrocław University of Environmental and Life Sciences, Chełmońskiego 37/41, 51-630 Wrocław, Poland
3Department of Vegetable Plant Protection, Research Institute of Horticulture in Skierniewice,
Konstytucji 3 Maja 1/3, 96-100 Skierniewice, Poland
Pol. J. Environ. Stud. 2013;22(4):1083–1090
KEYWORDS
ABSTRACT
Our study investigated the growth and sporulation of Trichoderma strains in solid-state cultures on lignocellulosic
materials and determined the survival and growth dynamics in bio-preparations after biomass drying
at 30ºC. All waste materials used in the study turned out to be good media for the production of
Trichoderma fungal biomass and the highest amount of colony-forming units per gram of dry matter (CFU/g
DM), and a number of conidia per gram of dry matter (conidia/g DM) were produced on wheat bran and sugar
beet pulp. The drying of biomass had no significant influence on the dynamics of fungal growth. Despite the
prolonging of the lag phase and reduction in the maximum specific growth rate, the biomass yield of the analyzed
strains was similar to the respective value noted in cultures before drying.
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