The main aim of this study was to describe the adsorption behaviours of acetaminophen (APAP) onto colloid and solid particle in sediment in terms of their impact on the factors of the processes (e.g., contact time, temperature, kinetic, thermodynamic characteristics) that were analyzed systematically. Sediment was collected from a typical tributary in the mid-Weihe River and a separation device was employed to separate sediment into colloid and solid particle. Results show that the adsorption of APAP onto colloid and solid particle reached equilibrium after about 12 hours and 48 hours, respectively. The adsorption capacity of APAP onto colloid was at least 28-fold larger than that onto solid particle with an initial APAP concentration of 10.0 mg·L^-1 at 25ºC. The Langmuir isotherm model was more suitable for describing the adsorption behaviours of APAP onto both colloid and solid particle in sediment. The adsorption processes were feasible, spontaneous, entropyincreasing, and endothermic in nature, the adsorption capacity increased with increasing temperature. The mechanisms controlling APAP adsorption onto colloid and solid particle were mainly physisorption, but physisorption less on APAP adsorption onto colloid. The obtained results provide a theoretical basis for the effective prevention of APAP pollution to the groundwater system in the Weihe River basin.