Plant secondary metabolites are rich source of medication ever since millennium time and can efficiently alienate biological actions. Aloe vera has been enchanted as a matchless cure for ailments globally ever since the historical epochs due to its ample biological actions. In the present study, ethanolic extract of A. vera leaves gel and its fractions (n-Hexane, Petroleum Ether, Chloroform, Dichloromethane, Acetone, Methanol and Aqueous) were being used for phytochemical analysis. In qualitative phytochemical exploration, the ethanolic extract and various fractions have shown the presence of numerous secondary metabolites including phenolics, flavonoids and alkaloids in all samples. In quantitative phytochemical investigation, the explicit appearance of the total phenolic, flavonoids and alkaloids content was recorded in methanol, dichloromethane and aqueous fractions. The ethanolic A. vera extract and its fractions were used to evaluate their antibacterial activity. A. vera ethanolic extract, methanol and aqueous fraction have exhibited greater antibacterial activity rather than other fractions. Thus, A. vera encompasses plentiful quantity of phytochemicals indicating its importance for exploitation as a herbal remedy for different diseases and as antibacterial agent.