The purpose of this research work was to detect antibiotic remnants from chicken meat and to quantify them. For this purpose, the chicken was collected from three poultry farms in Lahore Sheikhupura area. These chickens were categorized according to quality (A, B and C grade) and on the basis of the day of collection (7^th, 21^st and 35^th Growth day). The qualitative analysis was done by agar well diffusion method against B. subtilis, P. aeruginosa, E. coli and S. aureus. Analysis was done for muscles, kidney and liver samples. The highest residual potential was observed for liver extract (7^th day) while the minimum for muscle extract (35^th day). The samples with the highest zones of inhibition against pathogens were analyzed by HPLC.