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ORIGINAL RESEARCH
Characteristics of Bacterial Communities
in Rhizosphere and Non-Rhizosphere Soil
of the Relict Plant Diplandrorchis sinica S. C. Chen
1
College of Forestry, Shenyang Agricultural University, Shenyang 110866, Liaoning, China
2
Key Laboratory of Forest Tree Genetics, Breeding and Cultivation of Liaoning Province, Shenyang 110866, Liaoning,
China
3
Liaoning Laotudingzi national nature reserve fushun administration, Xinbin 113208, Liaoning, China
4
College of Life Science and Bioengineering, Shenyang Institute of Technology, Shenyang 113122, Liaoning, China
5
Liaoning Provincial Forestry and Grassland Bureau, Shenyang 110001, Liaoning, China
6
College of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang 110866, Liaoning, China
Submission date: 2023-08-24
Final revision date: 2024-02-28
Acceptance date: 2024-06-30
Online publication date: 2024-11-18
Publication date: 2025-07-05
Corresponding author
Xuhui Chen
College of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang 110866, Liaoning, China, China
College of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang 110866, Liaoning, China, China
Pol. J. Environ. Stud. 2025;34(5):5443-5454
KEYWORDS
Diplandrorchis sinicaRhizosphere soil microorganismsHigh-throughput sequencingBacterial diversityOrchidaceae
TOPICS
ABSTRACT
Diplandrorchis sinica is a monotypic orchid relict plant. Soil habitat and soil microorganisms
are the main factors for the growth and development of orchid species. In order to find out the soil
physical and chemical properties and soil microbial community characteristics in the growing area
of D. sinica and expand the population of D. sinica, the rhizosphere and non-rhizosphere soils
in the Rare and Endangered Species Reserve of Fushun, Laotudingzi Nature Reserve, Liaoning
Province, were collected for Illumina Miseq high-flux sequencing. The Alpha and Beta diversity
and LEfSe of the sequencing data were analyzed, combined with the physical and chemical properties
of the soil in the growth area of D. sinica to study the changes of soil physical and chemical properties,
soil bacterial composition and diversity in the growing area of D. sinica. The results showed that a total
of 26,690 valid bacterial sequences were obtained by sequencing. After cluster analysis, 9,556 OTUs
were classified into 34 phyla, 108 classes, 316 families, and 472 genera. Soil bacterial diversity is
rich in D. sinica, and the main dominant bacteria in rhizosphere and non-rhizosphere soil were
Proteobacteria, Bacteroidetes, Acidobacteria and Verrucomicrobia; at the level of order classification,
Rhizobiales and Gaiellales were mainly rhizobiales; at genus classification level, Hyphomicrobium
and Rhizobium were the main genera. The values of total nitrogen (STN), available phosphorus (AP),
hydrolyzed nitrogen (HN), total phosphorus (TP), total potassium (TK), and pH in rhizosphere soil
of D. sinica were significantly different from those in non-rhizosphere soil. Moreover, it was significantly
correlated with Proteobacteria, Actinobacteria, and Verrucomicrobia in soil microorganisms.
The soil physical and chemical properties affected the microbial and bacterial richness in rhizosphere
soil of Diactylodes. LEfSE analysis showed that 13 indicator species were selected when the alpha level was LDA > 2 and p < 0.05. The indicator species of Rhizo were Yersinia, Thermogemmatisporaceae,
Thermogemmatisporales, Spirosoma, etc. Bulk is mainly Merhylophilaceae, Methylophilales,
Chromatiales, etc. This study revealed for the first time the diversity of rhizosphere and non-rhizosphere
soil bacterial communities of the endangered species D. sinica, laying a foundation for the research on
the mechanism of the endangered species.
CONFLICT OF INTEREST
The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
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| eISSN: | 2083-5906 |
| ISSN: | 1230-1485 |
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