About the Journal
In Memory of Professor Jerzy Radecki
Ownership and Management Statement
Editorial Office
Editorial Board
Scope
Impact Factor
Indexed by
Journal Impact Factor contributing items
Publication Frequency
Revenue Sources & Business Model
Contact
Subscription
Current issue
Online first
Instructions for Authors
Copyright & Licensing
Publication Fees
Editorial policies
Publication Policy Overview
Peer Review Policy
Research Ethics and Malpractice Policy
Plagiarism Policy
Conflict of Interest Policy
Open Access Policy
Instructions for Reviewers
Generative AI and AI-Assisted Technologies Policy
Advertising Policy
Direct Marketing & Manuscript Solicitation
Digital Archiving and Preservation Policy
CrossMark, Correction, and Retraction Policy
Archive
Publication Fees
Copyright & Licensing
Editorial policies
Publication Policy Overview
Peer Review Policy
Research Ethics and Malpractice Policy
Plagiarism Policy
Conflict of Interest Policy
Open Access Policy
Instructions for Reviewers
Generative AI and AI-Assisted Technologies Policy
Advertising Policy
Direct Marketing & Manuscript Solicitation
Digital Archiving and Preservation Policy
CrossMark, Correction, and Retraction Policy
eISSN: 2083-5906
ISSN: 1230-1485
ISSN: 1230-1485
ORIGINAL RESEARCH
Eco-Friendly Approach for Synthesis of Silver
Nano-Particles Using Aqueous Leaf Extract
of Kalanchoe pinnata as Antimicrobial Agents
1
Department of Biotechnology, Graphic Era (Deemed to be University), Dehradun, 248002 Uttarakhand, India
2
Department of Research and Innovation, Uttaranchal University, Dehradun, 248007 Uttarakhand, India
3
Department of Earth and Environmental Sciences, Department of Microbiology and Molecular Genetics,
Michigan State University, East Lansing, MI, United States
4
Department of Microbiology, Graphic Era (Deemed to be University), Dehradun, 248002 Uttarakhand, India
5
Department of Food Science and Nutrition, College of Sciences, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia
6
Department of Biology, Turabah University College, Taif University, 21995, Saudi Arabia
7
Department of Biological Sciences, College of Science, University of Jeddah, Jeddah 21959, Saudi Arabia
8
Department of Physics, Turabah Branch, Turabah University College, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia
9
Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences,
University of Tabuk, Tabuk 71491, Saudi Arabia
Submission date: 2024-04-06
Final revision date: 2024-07-17
Acceptance date: 2024-08-03
Online publication date: 2024-10-21
Publication date: 2025-08-20
Corresponding author
Somya Sinha
Department of Biotechnology, Graphic Era (Deemed to be University), Dehradun, 248002 Uttarakhand, India
Department of Biotechnology, Graphic Era (Deemed to be University), Dehradun, 248002 Uttarakhand, India
Debasis Mitra
Department of Microbiology, Graphic Era (Deemed to be University), Dehradun, 248002 Uttarakhand, India
Department of Microbiology, Graphic Era (Deemed to be University), Dehradun, 248002 Uttarakhand, India
Rokayya Sami
Department of Food Science and Nutrition, College of Sciences, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia
Department of Food Science and Nutrition, College of Sciences, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia
Pol. J. Environ. Stud. 2025;34(5):6163-6176
KEYWORDS
TOPICS
ABSTRACT
The utilization of plant extracts in the biogenic amalgamation of metallic silver nano-particles is
a nature-friendly approach compared to the classical methods adopted earlier. The current study involved
the bio-synthesis of AgNPs using Kalanchoe pinnata leaf extract. The presence of phyto-constituents
in the leaf extract revealed the reducing ability of Kp-AgNPs followed by nucleation. Physicochemical
characterization techniques viz. scanning electron microscope (SEM), Fourier-transform infrared
spectroscopy (FTIR), transmission electron microscopy (TEM), UV-vis spectroscopy, electron
diffraction studies, and zeta potential gave spectra at 400 nm with surface morphological features
in the form of spherical AgNPs, detection of functional groups, and interaction between the particles
in the aqueous leaf extract. Moreover, Kp-AgNPs were effective in inhibiting different bacterial strains.
Hence, it can be inferred from this study that AgNPs have an inclusive range of applications, including
the fabrication of biomedical, optical, and electronic devices.
CONFLICT OF INTEREST
The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
REFERENCES (61)
1.
KHOJAH E., SAMI R., HELAL M., ELHAKEM A., BENAJIBA N., ALHARBI M., ALKALTHAM M.S. Effect of Coatings Using Titanium Dioxide Nanoparticles and Chitosan Films on Oxidation during Storage on White Button Mushroom. Crystals. 11, 1, 2021. https://doi.org/10.3390/cryst1....
2.
MALIK S., MUHAMMAD K., WAHEED Y. Nanotechnology: A revolution in modern industry. Molecules. 28 (2), 661, 2023. https://doi.org/10.3390/molecu....
3.
GIRI A.K., JENA B., BISWAL B., PRADHAN A.K., ARAKHA M., ACHARYA S., ACHARYA L. Green synthesis and characterization of silver nanoparticles using Eugenia roxburghii DC. extract and activity against biofilm-producing bacteria. Scientific Reports. 12 (1), 8383, 2022. https://doi.org/10.1038/s41598....
4.
GURUNATHAN S. Rapid biological synthesis of silver nanoparticles and their enhanced antibacterial effects against Escherichia fergusonii and Streptococcus mutans. Arabian Journal of Chemistry. 12 (2), 168, 2019. https://doi.org/10.1016/j.arab....
5.
XU L., WANG Y.Y., HUANG J., CHEN C.Y., WANG Z.X., XIE H. Silver nanoparticles: Synthesis, medical applications and biosafety. Theranostics. 10 (20), 8996, 2020. https://doi.org/10.7150/thno.4....
6.
DHAKA A., MALI S.C., SHARMA S., TRIVEDI R. A review on biological synthesis of silver nano-particles and their potential applications. Results in Chemistry. 6, 101108, 2023. https://doi.org/10.1016/j.rech....
7.
GUDIKANDULA K., CHARYA M.S. Synthesis of silver nanoparticles by chemical and bio-logical methods and their antimicrobial properties. Journal of Experimental Nanoscience. 11 (9), 714, 2016. https://doi.org/10.1080/174580....
8.
MBA I.E., NWEZE E.I. Nanoparticles as therapeutic options for treating multidrug-resistant bacteria: Research progress, challenges, and prospects. World Journal of Microbiology and Biotechnology. 37, 1, 2021. https://doi.org/10.1007/s11274....
9.
AFLAKIAN F., MIRZAVI F., AIYELABEGAN H.T., SOLEIMANI A., NAVASHENAQ J.G., KARIMI-SANI I., ZOMORODI A.R., VAKILI-GHARTAVOL R. Nanoparticles-based therapeutics for the management of bacterial infections: a special emphasis on FDA approved products and clinical trials. European Journal of Pharmaceutical Sciences. 188, 106515, 2023. https://doi.org/10.1016/j.ejps....
10.
ADENIJI O.O., NONTONGANA N., OKOH J.C., OKOH A.I. The potential of antibiotics and nano-material combinations as therapeutic strategies in the management of multidrug-resistant infections: a review. International Journal of Molecular Sciences. 23 (23), 15038, 2022. https://doi.org/10.3390/ijms23....
11.
MAMUN M.M., SORINOLU A.J., MUNIR M., VEJERANO E.P. Nanoantibiotics: Functions and properties at the nanoscale to combat antibiotic resistance. Frontiers in Chemistry. 9, 687660, 2021. https://doi.org/10.3389/fchem.....
12.
MORADI F., GHAEDI A., FOOLADFAR Z., BAZRGAR A. Recent advance on nanoparticles or nano-materials with anti-Multidrug resistant Bacteria and anti-bacterial biofilm properties; A systematic review. Heliyon. 9 (11), e22105, 2023. https://doi.org/10.1016/j.heli....
13.
YILMAZ G.E., GÖKTÜRK I., OVEZOVA M., YILMAZ F., KILIÇ S., DENIZLI A. Antimicrobial nanomaterials: a review. Hygiene. 3 (3), 269, 2023. https://doi.org/10.3390/hygien....
14.
CHANDRAKALA V., ARUNA V., ANGAJALA G. Review on metal nanoparticles as nanocarriers: Current challenges and perspectives in drug delivery systems. Emergent Materials. 5 (6), 1593, 2022. https://doi.org/10.1007/s42247....
15.
AHMAD S.A., DAS S.S., KHATOON A., ANSARI M.T., AFZAL M., HASNAIN M.S., NAYAK A.K. Bactericidal activity of silver nanoparticles: A mechanistic review. Materials Science for Energy Technologies. 3, 756, 2020. https://doi.org/10.1016/j.mset....
16.
DAS B., DASH S.K., MANDAL D., GHOSH T., CHATTOPADHYAY S., TRIPATHY S., DAS S., DEY S.K., DAS D., ROY S. Green synthesized silver nanoparticles destroy multidrug resistant bacteria via reactive ox-ygen species mediated membrane damage. Arabian Journal of Chemistry. 10 (6), 862, 2017. https://doi.org/10.1016/j.arab....
17.
RAY M.K., MISHRA A.K., MOHANTA Y.K., MAHANTA S., CHAKRABARTTY I., KUNGWANI N.A., AVULA S.K., PANDA J., PUDAKE R.N. Nanotechnology as a promising tool against phytopathogens: A futuristic approach to agriculture. Agriculture. 13 (9), 1856, 2023. https://doi.org/10.3390/agricu....
18.
BARROS C.H., FULAZ S., STANISIC D., TASIC L. Biogenic nanosilver against multidrug-resistant bacteria (MDRB). Antibiotics. 7 (3), 69, 2018. https://doi.org/10.3390/antibi....
19.
XIE J., LEE J.Y., WANG D.I., TING Y.P. Silver nanoplates: from biological to biomimetic synthesis. ACS Nano. 1 (5), 429, 2007. https://doi.org/10.1021/nn7000....
20.
GAUTAM D., DOLMA K.G., KHANDELWAL B., GUPTA M., SINGH M., MAHBOOB T., TEOTIA A., THOTA P., BHATTACHARYA J., GOYAL R., OLIVEIRA S.M.R., PEREIRA M.D.L., WIART C., WILAIRATANA P., EAWSAKUL K., RAHMATULLAH M., SARAVANABHAVAN S.S., NISSAPATORN V. Green synthesis of silver nanoparticles using Ocimum sanctum Linn. and its antibacterial activity against multidrug resistant Acinetobacter baumannii. PeerJ. 11, 2023. https://doi.org/10.7717/peerj.....
21.
XU Z., DENG M. Identification and Control of Common Weeds: Volume 2, Identif. Control Common Weeds. 2 (2), 475, 2017. https://doi.org/10.1007/978-94....
22.
GUPTA R., LOHANI M., ARORA S. Anti-inflammatory activity of the leaf extracts/ fractions of Bryophyllum pinnatum Saliv. International Journal of Pharmaceutical Sciences Review and Research. 3, 16, 2010.
23.
EKPO J.C., UDO E.S., TOM E.J., ARCHIBONG A.M., INYANG I.P. Effects of ethanolic and aqueous leaf extracts of Bryophyllum pinnatum on haematological parameters of normal and streptozotocin-induced diabetic rats. Biokemistri. 33 (3), 181, 2021.
24.
YADAV M., GULKARI V., WANJARI M. Bryophyllum pinnatum leaf extracts prevent formation of renal calculi in lithiatic rats. Ancient Science of Life. 36, 90, 2016. https://doi.org/10.4103/asl.AS....
25.
SOHGAURA A., BIGONIYA P., SHRIVASTAVA B. Invitro antilithiatic potential of Kalanchoe pinnata, Emblica officinalis, Bambusa nutans, and Cynodon dactylon. Journal of Pharmacy and Bioallied Sciences. 10, 83, 2018. https://doi.org/10.4103/JPBS.J....
26.
RAFIQUE M., SADAF I., RAFIQUE M.S., TAHIR M.B. A review on green synthesis of AgNPs and their applications. Artif. Cells, Nanomed. Biotechnol. 45, 1272, 2017. https://doi.org/10.1080/216914....
27.
MARCINIAK L., NOWAK M., TROJANOWSKA A., TYLKOWSKI B., JASTRZAB R. The effect of pH on the size of silver nanoparticles obtained in the reduction reaction with citric and malic acids. Materials. 13 (23), 5444, 2020. https://doi.org/10.3390/ma1323....
28.
HANDAYANI W., NINGRUM A.S., IMAWAN C. The role of pH in synthesis silver nanoparticles using Pometia pinnata (matoa) leaves extract as bioreductor. Journal of Physics: Conference Series. 1428 (1), 012021, 2020. https://doi.org/10.1088/1742-6....
29.
RAJAN R., CHANDRAN K., HARPER S.L., YUN S.I., KALAICHELVAN P.T. Plant extract synthesized silver nanoparticles: An ongoing source of novel biocompatible materials. Industrial Crops and Products. 70, 356, 2015. https://doi.org/10.1016/j.indc....
30.
ANTUNES F.S., DOS S.S., DOS A.L., BACKX B.P., SORAN M.L., OPRIŞ O., LUNG I., STEGARESCU A., BOUOUDINA M. Biosynthesis of nanoparticles using plant extracts and essential oils. Molecules. 28 (7), 3060, 2023. https://doi.org/10.3390/molecu....
31.
VANLALVENI C., LALLIANRAWNA S., BISWAS A., SELVARAJ M., CHANGMAI B., ROKHUM S.L. Green synthesis of silver nanoparticles using plant extracts and their antimicrobial activities: A review of recent literature. RSC Advances. 11 (5), 2804, 2021. https://doi.org/10.1039/D0RA09....
32.
JHA S.K., JHA A. Plant Extract Mediated Synthesis of Metal Nanoparticles, their Characterization and Applications: A Green Approach. Current Green Chemistry. 8 (3), 185, 2021. https://doi.org/10.2174/221334....
33.
SIAKAVELLA I.K., LAMARI F., PAPOULIS D., ORKOULA M., GKOLFI P., LYKOURAS M., AVGOUSTAKIS K., HATZIANTONIOU S. Effect of plant extracts on the characteristics of silver nanoparticles for topical application. Pharmaceutics. 12 (12), 1244, 2020. https://doi.org/10.3390/pharma....
34.
IBRAHIM N.H., TAHA G.M., HAGAGGI N.S.A., MOGHAZY M.A. Green synthesis of silver nanoparticles and its environmental sensor ability to some heavy metals. BMC Chemistry. 18 (1), 7, 2024. https://doi.org/10.1186/s13065....
35.
ANSARI M., AHMED S., ABBASI A., KHAN M.T., SUBHAN M., BUKHARI N.A., HATAMLEH A.A., ABDELSALAM N.R. Plant mediated fabrication of silver nanoparticles, process optimization, and impact on tomato plant. Scientific Reports. 13 (1), 18048, 2023. https://doi.org/10.1038/s41598....
36.
VIJAYAKUMAR S., MALAIKOZHUNDAN B., SARAVANAKUMAR K., DURÁN-LARA E.F., WANG M.H., VASEEHARAN B. Garlic clove extract assisted silver nanoparticle - Antibacterial, antibiofilm, antihelminthic, anti-inflammatory, anticancer and ecotoxicity assessment. Journal of Photochemistry and Photobiology B: Biology. 198, 111558, 2019. https://doi.org/10.1016/j.jpho....
37.
JAIN S., MOHAN S.M. Medicinal plant leaf extract and pure flavonoid mediated green synthesis of AgNPs and their enhanced antibacterial property. Scientific Reports. 7 (1), 1, 2017. https://doi.org/10.1038/s41598....
38.
BIANCHINI F.R., DOMINGUES R.J., LEMOS B.T.W., GONCALVES G.A.D., DE P.F., PRATAVIEIRA S., CHIAVACCI L.A., JUNIOR J.P.A., COSTA P.I.D., MARTINEZ L.R. Zinc-based nanoparticles reduce bacterial biofilm formation. Microbiology Spectrum. 11 (2), e04831, 2023. https://doi.org/10.1128/spectr....
39.
NAIDU S., SINGH I.K., SINGH A. Microbial Synthesis of Magnetic Nanoparticles for Plant Science and Agriculture. Plant Nano Biology. 4, 100036, 2023. https://doi.org/10.1016/j.plan....
40.
DIPIETRO R.S., JOHNSON H.G., BENNETT S.P., NUMMY T.J., LEWIS L.H., HEIMAN D. Determining magnetic nanoparticle size distributions from thermomagnetic measurements. Applied Physics Letters. 96 (22), 222506, 2010. https://doi.org/10.1063/1.3441....
41.
SAMI R., KHOJAH E., ELHAKEM A., BENAJIBA N., HELAL M., ALHUTHAL N., ALZAHRANI S.A., ALHARBI M., CHAVALI M. Performance Study of Nano/SiO2 Films and the Antimicrobial Application on Cantaloupe Fruit Shelf-Life. Applied Sciences. 11, 1, 2021. https://doi.org/10.3390/app110....
42.
RAJA S., RAMESH V., THIVAHARAN V. Green biosynthesis of AgNPs using Calliandra haematocephala leaf extract, their antibacterial activity and hydrogen peroxide sensing capability. Arabian Journal of Chemistry. 10, 253, 2017. https://doi.org/10.1016/j.arab....
43.
IMALI A.M., ALAA A.M., VICKI H.G. ATR-FTIR Spectroscopy as a Tool to Probe Surface Adsorption on Nanoparticles at the Liquid-Solid Interface in Environmentally and Biologically Relevant Media. Analyst. 139 (5), 654, 2014. https://doi.org/10.1039/C3AN01....
44.
WAN W.K.A., SHAMELI K., JAZAYERI S.D., OTHMAN N.A., CHE N.W., HASSAN N.M. Biosynthesized silver nanoparticles by aqueous stem extract of Entada spiralis and screening of their biomedical activity. Frontiers in Chemistry. 8, 620, 2020. https://doi.org/10.3389/fchem.....
45.
AL-ZAHRANI S., ASTUDILLO-CALDERÓN S., PINTOS B., PÉREZ-URRIA E., MANZANERA J.A., MARTÍN L., GOMEZ-GARAY A. Role of synthetic plant extracts on the production of silver-derived nanoparticles. Plants. 10 (8), 1671, 2021. https://doi.org/10.3390/plants....
46.
RAMON P., BERGMANN D., ABDULLA H., SPARKS J., OMORUYI F. Bioactive ingredients in K. pinnata extract and synergistic effects of combined K. pinnata and metformin preparations on antioxidant activities in diabetic and non-diabetic skeletal muscle cells. International Journal of Molecular Sciences. 24 (7), 6211, 2023. https://doi.org/10.3390/ijms24....
47.
VILLARREAL W.L., ROBLES J.E., COSTA G.M. Phytochemical Standardization of an Extract Rich in Flavonoids from Flowers of Kalanchoe pinnata (Lam) Pers. Scientia Pharmaceutica. 91 (4), 50, 2023. https://doi.org/10.3390/scipha....
48.
KENDESON C.A., KAGORO M.L., ADELAKUN E.A. Phytochemical and pharmacological evaluation of Nigerian Kalanchoe pinnata (Lam.) stem-bark. Journal of Chemical Society of Nigeria. 46 (4), 2021. https://doi.org/10.46602/jcsn.....
49.
GHASEMPOUR A., DEHGHAN H., ATAEE M., CHEN B., ZHAO Z., SEDIGHI M., GUO X., SHAHBAZI M.A. Cadmium sulfide nanoparticles: preparation, characterization, and biomedical applications. Molecules. 28 (9), 3857, 2023. https://doi.org/10.3390/molecu....
50.
MAHMOUDI M. The need for robust characterization of nanomaterials for nanomedicine applications. Nature Communications. 12 (1), 5246, 2021. https://doi.org/10.1038/s41467....
51.
LIU H., ZHANG H., WANG J., WEI J. Effect of temperature on the size of biosynthesized silver nanoparticle: deep insight into microscopic kinetics analysis. Arabian Journal of Chemistry. 13 (1), 1011, 2020. https://doi.org/10.1016/j.arab....
52.
AMALA S.E., NWEKE S.N., NWALOZIE R., MONSI T.P. Antimicrobial properties and phytochemical composition of Garcinia kola, Bryophyllum pinnatum, and Allium sativum juices on some clinical pathogens. Advances in Bioscience and Biotechnology. 12 (11), 388, 2021. https://doi.org/10.4236/abb.20....
53.
LIAO C., LI Y., TJONG S.C. Bactericidal and cytotoxic properties of silver nanoparticles. International Journal of Molecular Sciences. 20 (2), 449, 2019. https://doi.org/10.3390/ijms20....
54.
HASSAN A., ULLAH H. Antibacterial and antifungal activities of the medicinal plant Veronica biloba. Journal of Chemistry. 5264943, 1, 2019. https://doi.org/10.1155/2019/5....
55.
URNUKHSAIKHAN E., BOLD B.E., GUNBILEG A., SUKHBAATAR N., MISHIG-OCHIR T. Antibacterial activity and characteristics of silver nanoparticles biosynthesized from Carduus crispus. Scientific Reports. 11 (1), 21047, 2021. https://doi.org/10.1038/s41598....
56.
PATRA J.K., BAEK K.H. Antibacterial activity and synergistic antibacterial potential of biosynthesized AgNPs against foodborne pathogenic bacteria along with its anticandidal and antioxidant effects. Frontiers in Microbiology. 8, 167, 2017. https://doi.org/10.3389/fmicb.....
57.
QING Y., CHENG L., LI R., LIU G., ZHANG Y., TANG X., WANG J., LIU H., QIN Y. Potential antibacterial mechanism of AgNPs and the optimization of orthopedic implants by advanced modification technologies. International Journal of Nanomedicine. 13, 3311, 2018. https://doi.org/10.2147/IJN.S1....
58.
ROZHIN A., BATASHEVA S., KRUYCHKOVA M., CHEREDNICHENKO Y., ROZHINA E., FAKHRULLIN R. Biogenic silver nanoparticles: Synthesis and application as antibacterial and antifungal agents. Micromachines. 12 (12), 1480, 2021. https://doi.org/10.3390/mi1212....
59.
SHAH M.Z., GUAN Z.H., DIN A.U., ALI A., REHMAN A.U., JAN K., FAISAL S., SAUD S., ADNAN M., WAHID F., ALAMRI S. Synthesis of silver nanoparticles using Plantago lanceolata extract and assessing their antibacterial and antioxidant activities. Scientific Reports. 11, 20754, 2021. https://doi.org/10.1038/s41598....
60.
KHAN S.U., KHAN M.S., WANG H., QIAN M., JAVED T., FAHAD S., LU K. Harnessing nanobiotechnology for drought stress: transforming agriculture's future; what, why and how? Environmental Science: Nano. 7, 2024. https://doi.org/10.1039/D4EN00....
61.
ABID M., KHALID N., QASIM A., SAUD A., MANZER H.S., CHAO W., DEPENG W., SHAH S., JAN B., SUBHAN D., RAHUL D. Exploring the potential of moringa leaf extract as bio stimulant for improving yield and quality of black cumin oil. Scientific Reports. 11, 24217, 2021. https://doi.org/10.1038/s41598....
CITATIONS (1):
1.
Eco-Friendly Synthesis, Characterization and Multifunctional Bioactivity of Phyto-Capped Zinc Oxide Nanoparticles Derived from Kalanchoe fedtschenkoi
Hrishikesh H. Khodade, Deepak B. Shelke, Hiralal B. Sonawane, Kamlakar C. More, Mahadev R. Chambhare
Asian Journal of Chemistry
Hrishikesh H. Khodade, Deepak B. Shelke, Hiralal B. Sonawane, Kamlakar C. More, Mahadev R. Chambhare
Asian Journal of Chemistry
| eISSN: | 2083-5906 |
| ISSN: | 1230-1485 |
We process personal data collected when visiting the website. The function of obtaining information about users and their behavior is carried out by voluntarily entered information in forms and saving cookies in end devices. Data, including cookies, are used to provide services, improve the user experience and to analyze the traffic in accordance with the Privacy policy. Data are also collected and processed by Google Analytics tool (more).
You can change cookies settings in your browser. Restricted use of cookies in the browser configuration may affect some functionalities of the website.
You can change cookies settings in your browser. Restricted use of cookies in the browser configuration may affect some functionalities of the website.
