Nitrogen losses of nitrous oxide (N₂O) in grazed grassland are strongly driven by urine deposition by grazing ruminants. There is robust concern about applying nitrification inhibitor to mitigate nitrogen losses in global grasslands. However, the effect of dicyandiamide (DCD) on N₂O emission rates and its driven factors remain unclear due to spatial heterogeneity. In the present study, we synthesized 133 group data from 69 published articles. It was indicated that effect size was -0.784±0.048 (P<0.0001) indicating a reduction of 54.34% in N₂O emission rates. There was significant difference on effect size between different dose of nitrogen fertilization. Moderate dosage of DCD application was the best mitigation effect of 56.09%, which was significantly higher than light dosage of DCD application. Mixed effect model results revealed that precipitation, bulk density and air temperature could explain 16.93%, 12.31% and 3.02% variations in effect size. In addition, DCD application was an effective strategy to mitigate N₂O emission rates in global grazed grasslands.