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ORIGINAL RESEARCH
Gasless Removal of Nitrogen by
a Phosphate-Accumulating Organism
Cupriavidus Plantarum S7–1A
1
Shenzhen Key Laboratory of Marine Bioresource & Eco-environmental Sciences, College of Life Sciences
and Oceanography, Shenzhen University, Shenzhen, 518071, China
2
College of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen 518060, China
Submission date: 2024-03-23
Final revision date: 2024-04-23
Acceptance date: 2024-05-19
Online publication date: 2024-10-29
Publication date: 2025-04-04
Corresponding author
Shuangfei Li
Shenzhen University, No. 1066 Xueyuan Avenue, Nanshan District, 518060, Shenzhen, China
Shenzhen University, No. 1066 Xueyuan Avenue, Nanshan District, 518060, Shenzhen, China
Pol. J. Environ. Stud. 2025;34(4):3593-3606
KEYWORDS
Cupriavidus plantarum S7–1APhosphate-accumulating organismAssimilatory nitrate reductionNitrogen removalNitrogen assimilation
TOPICS
ABSTRACT
A highly efficient phosphorus-accumulating organism, Cupriavidus plantarum S7–1A, which
can completely remove inorganic nitrogen, was isolated from municipal activated sludge. The S7–1A
generated no gaseous nitrogen, was confirmed to produce no hemolysin, and showed high susceptibility
to cephalosporin and floxacin antibiotics. Optimum conditions were obtained with sodium pyruvate
as a carbon source, a C/N of 18, a pH of 7–9, a temperature of 30°C, a phosphorus concentration
of 16.5 mg/L, and shaking at 250 rpm. The highest nitrate, nitrite, and ammonium removal efficiencies
were respectively 99.94%, 99.98%, and 99.62%, and the corresponding removal rates were 4.87, 3.84,
and 6.90 mg/L/h. Over 89% of total organic carbon was removed, and phosphorus was not detected at
initial concentrations below 16.5 mg/L. Genome sequencing and PCR confirmed that S7–1A has nasA,
nirB, nirD, ppk, ppk2, and ppx genes. In conclusion, S7–1A proved to be a safe and efficient assimilatory
nitrate reduction and phosphorus-accumulating organism (ANR-PAO).
CONFLICT OF INTEREST
The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
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| eISSN: | 2083-5906 |
| ISSN: | 1230-1485 |
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