This study aimed to investigate the antimicrobial resistance and virulence factors of Enterococci isolated from seawater using both phenotypic and genotypic methods. A comparison of the phenotypic virulence factors between Enterococcus faecalis and Enterococcus faecium strains revealed that gelatinase activity was 91.3% and 89.5%, respectively. Caseinase activity was observed at rates of 65.2% for E. faecalis and 31.5% for E. faecium. The capacity for strong adhesion in biofilm formation was 41.3% in E. faecalis and 42.1% in E. faecium. Serum resistance activity was noted at 54.3% for E. faecalis and 31.6% for E. faecium. Remarkably, hemagglutination activity showed a strong activity in 39.1% of E. faecalis strains, while E. faecium exhibited 0% activity. Antibacterial activity against Staphylococcus aureus ATCC 25923 was 32.6% for E. faecalis and 5.3% for E. faecium, and against Escherichia coli ATCC 25922, the activity was 23.9% and 0%, respectively. It was determined that one E. faecalis strain (2.17%) and one Enterococcus gallinarum strain (100%) contained at least four resistance genes. Additionally, one E. faecalis strain (2.17%) harbored up to 11 of the 12 tested antibiotic resistance genes. Notably, two E. faecalis strains (3.03%) exhibited the most virulent characteristics, encompassing 12 virulence gene regions. In contrast, one E. gallinarum strain (100%) manifested the least virulent characteristics, comprising three virulence gene regions. These findings indicate that marine Enterococci could pose a public health threat, necessitating ongoing surveillance.