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eISSN: 2083-5906
ISSN: 1230-1485
ISSN: 1230-1485
ORIGINAL RESEARCH
Nitrification and Urease Inhibitors Reduce
the Stimulated Nitrous Oxide Emissions
by the Freeze-Thaw Cycles
1
Key Laboratory of Southwest China Wildlife Resource Conservation (Ministry of Education).
China West Normal University, Nanchong, 637009, P.R. China
2
State Key Laboratory of Highland Barley and Yak Germplasm Resources and Genetic Improvement,
Lhasa, 850000, P.R. China
3
Institute of Pratacultural Science, Tibet Academy of Agriculture and Animal Husbandry Science,
Lhasa, 850000, P.R. China
4
Gansu Provincial Institute of Agricultural Engineering and Technology, Wuwei,733006, P.R. China
These authors had equal contribution to this work
Submission date: 2023-10-27
Final revision date: 2023-12-07
Acceptance date: 2024-03-05
Online publication date: 2024-07-08
Publication date: 2025-01-02
Corresponding author
Junqiang Wang
Key Laboratory of Southwest China Wildlife Resource Conservation (Ministry of Education). China West Normal University, China
Key Laboratory of Southwest China Wildlife Resource Conservation (Ministry of Education). China West Normal University, China
Pol. J. Environ. Stud. 2025;34(1):347-358
KEYWORDS
TOPICS
ABSTRACT
Most studies have demonstrated that nitrification and urease inhibitors can reduce soil nitrous oxide
(N2O) emissions from nitrogen-fertilized farmland. However, few studies have examined the potential
impacts of these inhibitors on semi-arid agricultural farmland in the presence of freeze-thaw (FT) cycles. The
purpose of this study was to assess the efficacy of applying the nitrification inhibitor 3,4-dimethylpyrazole
phosphate (DMPP) and the urease inhibitor N-(n-butyl) thiophosphoric triamide (NBPT) to soil nitrogen
transformation and studying N2O emissions through simulated indoor FT incubation to offer theoretical
and technological guidance for mitigating nitrogen loss in semi-arid farmland. The results showed that
urea with DMPP under freeze-thaw conditions significantly increased the inorganic nitrogen content of the
soil, kept the ammonium nitrogen content of the soil at a high level, suppressed the net nitrification rate of
the soil, and reduced the cumulative emission of nitrous oxide (N2O) in the soil by nearly 87.6% compared
to CK. Urea incubation with NBPT under freeze-thaw conditions also significantly reduced fluxes and
cumulative N2O emissions. Due to the dual inhibition of soil nitrification rate by DMPP/NBPT and the FT
cycle, the addition of DMPP/NBPT during soil FT could alleviate soil N2O emission caused by the effect
of the FT cycle after urea addition and reduce soil nitrogen loss. The results indicate that the application of
DMPP/NBPT can effectively alleviate the irrigated silt soil N2O emission during the FT period.
CONFLICT OF INTEREST
The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
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