New types of dyestuff moieties are being introduced to get the ease in imparting color to various substrates and sorting out the ways to improve the quality of dyed fabric using environmentally friendly techniques. To contribute to this research effort, the present study was carried for dyeing cellulosic fabric using novel bi-functional reactive dye. The synthesized dye contained sulphatovinylsulfone and monochlorotriazine functionality. The important influencing parameters for the exhaust method, e.g., dyeing temperature, concentration of salt, and alkali were optimized and their interaction was studied using central composite design. The results indicated that 61.5ºC was found to be suitable for the maximum exhaustion and fixation of the dye on cotton fabric. The optimum concentration of salt (30.01 g/L) and alkali (22.32 g/L) for dye experimentally resulted in 84.98 (±3)% of exhaustion and 79.00 (±3)% fixation on cellulose fabric. Fastness properties were good-to-excellent for the novel reactive dye applied on cotton fabric. A comparison of the results with the commercial reactive dye showed that attained results were within a practical range suitable for commercial dyeing.